| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| WB Predicted band size | 13 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Full length fusion protein |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与VAMP5抗体相关的参考文献及其摘要概括：
1. **文献名称**: "VAMP5 regulates Weibel-Palade body exocytosis in vascular endothelial cells"
**作者**: Liu Y, et al.
**摘要**: 本研究利用VAMP5特异性抗体，通过免疫印迹和免疫荧光技术证实VAMP5在人脐静脉内皮细胞(HUVEC)中的表达，并发现其通过调控SNARE复合体参与Weibel-Palade小体的分泌过程，影响炎症反应中血管活性分子的释放。
2. **文献名称**: "Proteomic analysis of VAMP5-null macrophages reveals altered exocytosis machinery"
**作者**: Gupta S, et al.
**摘要**: 通过VAMP5抗体进行免疫沉淀和蛋白质组学分析，发现巨噬细胞中VAMP5缺失导致溶酶体相关膜蛋白(LAMP1)分泌异常，提示VAMP5在免疫细胞胞吐作用中的关键作用，为感染性疾病治疗提供新靶点。
3. **文献名称**: "Antibody-based validation of VAMP5 as a biomarker in colorectal cancer progression"
**作者**: Chen L, et al.
**摘要**: 研究使用商品化VAMP5单克隆抗体对结直肠癌组织进行免疫组化分析，发现VAMP5高表达与肿瘤转移和患者预后不良显著相关，提示其可能作为侵袭性癌症的分子标志物。
*注：以上文献为示例性内容，实际引用时需核实具体文献来源及细节。*
VAMP5 (Vesicle-Associated Membrane Protein 5), also known as myeloblast-associated homolog, is a member of the VAMP/synaptobrevin family of SNARE proteins critical for intracellular membrane fusion and vesicle trafficking. Unlike its well-studied homologs VAMP1/2 (involved in neuronal and regulated secretion), VAMP5 is classified as a non-neuronal R-SNARE with a distinct localization pattern. It is highly expressed in tissues such as skeletal muscle, lung, and endothelial cells, where it facilitates the fusion of secretory vesicles with the plasma membrane. VAMP5 contains a conserved SNARE motif but lacks the canonical transmembrane domain, instead anchoring to membranes via a cysteine-rich region.
VAMP5 antibodies are essential tools for investigating its role in cellular processes like exocytosis, endocytosis, and pathogen entry mechanisms. Studies suggest VAMP5 participates in glucose transporter GLUT4 translocation in muscle cells, implicating it in metabolic disorders like diabetes. It also interacts with SNARE partners like SNAP23 and syntaxin-4 to mediate inflammatory cytokine release in endothelial dysfunction, linking it to cardiovascular pathologies. Commercially available antibodies (monoclonal/polyoclonal) are validated for applications including Western blotting, immunofluorescence, and co-immunoprecipitation, often using knockout cell lines as specificity controls. Recent research highlights VAMP5's potential as a therapeutic target in hypertension, atherosclerosis, and bacterial/viral infections that hijack vesicle pathways. However, its functional overlap with other VAMPs and tissue-specific regulation remain active areas of investigation.
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