| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/150-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | HLUG25; UDPGTH1; UGT2B11; UDPGT2B4; UDPGTh-1 |
| WB Predicted band size | 61 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human UGT2B4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与UGT2B4抗体相关的文献概览：
1. **"Characterization of UDP-glucuronosyltransferase 2B4 in human liver"**
- **作者**: Radominska-Pandya, A. 等
- **摘要**: 研究通过特异性抗体分析UGT2B4在肝脏组织中的表达模式，揭示其在胆汁酸和类固醇代谢中的功能，并验证抗体的免疫检测适用性。
2. **"Substrate specificity of human UGT2B4 and development of a monoclonal antibody for its detection"**
- **作者**: Jinno, H. 等
- **摘要**: 开发针对UGT2B4的单克隆抗体，用于Western blot和免疫组化，证实其在乳腺癌及肝细胞中的差异表达，并探讨其底物代谢特异性。
3. **"Monoclonal antibodies against human UDP-glucuronosyltransferases: Tools for phenotyping isoform-specific expression"**
- **作者**: Girard, B. 等
- **摘要**: 报道多种UGT家族抗体的制备，包括UGT2B4抗体，用于检测不同组织中的蛋白表达水平及酶活性关联分析。
4. **"Genetic polymorphisms and functional characterization of UGT2B4 variants in drug metabolism"**
- **作者**: Mackenzie, P.I. 等
- **摘要**: 利用特异性抗体研究UGT2B4基因多态性对酶表达的影响，揭示其与药物代谢个体差异的潜在关联。
(注：以上文献信息为模拟示例，实际引用需根据具体论文核实。)
The UDP-glucuronosyltransferase 2B4 (UGT2B4) enzyme, part of the UGT superfamily, plays a critical role in phase II metabolism by conjugating glucuronic acid to endogenous compounds (e.g., bile acids, steroid hormones) and xenobiotics, enhancing their solubility for excretion. UGT2B4 is predominantly expressed in the liver, gastrointestinal tract, and steroid-responsive tissues, with genetic polymorphisms influencing enzyme activity and interindividual variability in drug metabolism and disease susceptibility.
UGT2B4-specific antibodies are essential tools for studying its expression, localization, and function in physiological and pathological contexts. These antibodies enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research on UGT2B4's role in hormone regulation, detoxification, and cancer progression (e.g., hormone-dependent cancers). Dysregulation of UGT2B4 has been linked to metabolic disorders, cholestasis, and resistance to therapies targeting steroid signaling. Commercially available antibodies are typically developed in rabbits or mice, targeting epitopes within the enzyme's variable substrate-binding regions. Validation includes specificity testing using knockout models or siRNA-mediated silencing. Reliable UGT2B4 antibodies are crucial for advancing pharmacokinetic studies, biomarker discovery, and personalized medicine approaches.
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