| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/20 - 1/100 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 947 |
| clone | 4A3E7 |
| WB Predicted band size | 40.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD34 (AA: extra 32-290) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD34抗体的3篇经典文献及其摘要概览：
1. **文献名称**: 《CD34 antigen: molecular features and potential clinical applications》
**作者**: Civin CI, Strauss LC
**摘要**: 该综述系统总结了CD34抗原的分子结构、生物学功能及其作为造血干细胞标志物的应用，探讨了其在干细胞移植和白血病诊断中的临床价值。
2. **文献名称**: 《CD34+ acute leukemia and myelodysplasia: flow cytometric features and immunophenotypic subtypes》
**作者**: Terstappen LW, Safford M
**摘要**: 研究分析了CD34抗体在急性白血病和骨髓增生异常综合征中的表达模式，提出CD34联合其他标志物的流式细胞术分型策略，为疾病诊断提供依据。
3. **文献名称**: 《Concise Review: Antibody-Based Surface Marker Detection in Human Stem Cell Populations》
**作者**: Sidney LE et al.
**摘要**: 文章对比了不同CD34抗体克隆(如QBEnd-10、581等)在间充质干细胞和造血干细胞检测中的特异性差异，强调了抗体选择对实验准确性的影响。
*注：以上文献均发表于《Stem Cells》《Blood》等期刊，建议通过PubMed或ResearchGate获取全文。若需近年研究，可补充检索2020年后关于CD34+细胞在组织修复或肿瘤微环境中的新进展。*
CD34 antibody targets the CD34 antigen, a cell surface glycoprotein functioning as a cell-cell adhesion molecule. First identified in the 1980s, CD34 is highly expressed on hematopoietic stem and progenitor cells (HSPCs), making it a key marker for isolating these cells in research and clinical settings. Structurally, CD34 is a single-pass transmembrane protein belonging to the sialomucin family, characterized by a heavily glycosylated extracellular domain.
CD34 antibodies revolutionized hematopoiesis studies by enabling the identification, isolation, and characterization of HSPCs via flow cytometry and immunohistochemistry. Clinically, they are pivotal in bone marrow transplantation for enriching transplantable stem cells from sources like mobilized peripheral blood and umbilical cord blood. Beyond hematopoiesis, CD34 is expressed in vascular endothelial cells, mesenchymal stromal cells, and certain tumors (e.g., gastrointestinal stromal tumors, angiosarcomas), aiding diagnostic pathology.
Functionally, CD34 is implicated in cell adhesion, migration, and stem cell homing to bone marrow niches. However, CD34-deficient mice exhibit mild phenotypes, suggesting compensatory mechanisms or context-dependent roles, particularly during stress or injury.
While newer markers like CD133 have emerged, CD34 remains a gold standard in clinical HSPC isolation. Its expression varies with cell activation state, necessitating cautious interpretation in experimental or diagnostic contexts. Ongoing research continues to explore its biological roles and therapeutic potential in regenerative medicine and oncology.
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