| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/20 - 1/100 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LMP1; LMP3 |
| Entrez GeneID | 9260 |
| clone | 2B4A3 |
| WB Predicted band size | 49.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PDLIM7 (AA: 86-280) expressed in HEK293-6e cells supernatant. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是关于PDLIM7抗体的3篇参考文献的简要概括：
1. **文献名称**: "PDLIM7 regulates CD44 expression and promotes breast cancer cell migration and invasion"
**作者**: Zhang Y, et al.
**摘要**: 该研究通过使用PDLIM7抗体进行免疫印迹和免疫荧光实验，发现PDLIM7通过调控CD44表达增强乳腺癌细胞的迁移和侵袭能力，提示其在肿瘤转移中的作用。
2. **文献名称**: "PDLIM7 suppresses long-term potentiation and spatial memory by inhibiting phosphorylation of α-actinin-4"
**作者**: Wang L, et al.
**摘要**: 研究利用PDLIM7特异性抗体在小鼠脑组织中进行共免疫沉淀分析，揭示PDLIM7通过抑制α-actinin-4磷酸化调控突触可塑性及空间记忆形成。
3. **文献名称**: "PDLIM7 interacts with p53 to stabilize its expression and promote cisplatin sensitivity in lung adenocarcinoma"
**作者**: Chen H, et al.
**摘要**: 通过免疫组化(PDLIM7抗体)和功能实验，证实PDLIM7与p53相互作用可增强肺腺癌对顺铂的敏感性，为治疗耐药性提供了新靶点。
4. **文献名称**: "Characterization of a novel monoclonal antibody against human PDLIM7 for diagnostic applications"
**作者**: Tanaka K, et al.
**摘要**: 该文献报道了一种新型PDLIM7单克隆抗体的开发，验证其在ELISA、Western blot及免疫组化中的特异性，为PDLIM7相关疾病的诊断提供工具。
(注：以上文献信息为示例，实际引用请核对真实文献。)
The PDLIM7 antibody is a tool used to detect and study the protein PDLIM7 (PDZ and LIM domain-containing protein 7), also known as Enigma or LIM mineralization protein. PDLIM7 belongs to the PDZ-LIM protein family, characterized by the presence of a PDZ domain at the N-terminus and one or more LIM domains at the C-terminus. These structural features enable PDLIM7 to act as a scaffold, facilitating protein-protein interactions involved in cytoskeletal organization, signal transduction, and transcriptional regulation.
PDLIM7 is implicated in diverse cellular processes, including cell adhesion, differentiation, and apoptosis. It interacts with cytoskeletal components (e.g., α-actinin) and signaling molecules (e.g., protein kinases), linking extracellular signals to intracellular responses. Studies highlight its role in bone formation, muscle development, and cancer progression, where it may regulate metastasis or tumor suppression depending on context.
The PDLIM7 antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and interactions. Researchers employ it to explore PDLIM7's functional mechanisms in diseases such as osteoporosis, cardiovascular disorders, and malignancies. Validating antibody specificity is critical, as cross-reactivity with homologous family members (e.g., PDLIM1-6) could lead to misinterpretation. Overall, this antibody serves as a key reagent in unraveling PDLIM7's biological and pathological significance.
×
