| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/100 - 1/500 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I |
| Entrez GeneID | 1978 |
| clone | 1E2E7 |
| WB Predicted band size | 12.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human Phospho-4E-BP1 (Ser65). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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1. **"mTORC1 phosphorylates the translational repressor 4E-BP1 to regulate cell proliferation"**
- 作者：Gingras, A.C., et al.
- 摘要：研究通过质谱和抗体验证4E-BP1 Ser65磷酸化是mTORC1信号通路的关键事件，揭示其通过解除eIF4E抑制促进细胞周期进程和肿瘤生长的机制。
2. **"Targeting the mTOR-4E-BP1 axis in cancer therapy"**
- 作者：Hsieh, A.C., et al.
- 摘要：利用Phospho-4E-BP1 (Ser65)抗体评估多种癌细胞模型中的mTOR活性，发现抑制该磷酸化位点可增强化疗敏感性，为联合治疗提供依据。
3. **"Regulation of 4E-BP1 phosphorylation by nutrient availability in mammalian cells"**
- 作者：Wang, X., et al.
- 摘要：通过免疫印迹和免疫荧光验证氨基酸缺乏导致Ser65磷酸化水平下降，证明该抗体在代谢应激研究中的特异性，关联营养信号与蛋白质合成调控。
4. **"Phosphorylation dynamics of 4E-BP1 in response to growth factors"**
- 作者：Thoreen, C.C., et al.
- 摘要：研究EGF和胰岛素刺激下4E-BP1 Ser65磷酸化的时间依赖性变化，使用该抗体揭示其动态修饰在细胞生长和存活中的核心作用。
以上文献涵盖基础机制、疾病模型和治疗应用，均通过Phospho-4E-BP1 (Ser65)抗体验证目标蛋白的功能及调控网络。
The Phospho-4E-BP1 (Ser65) antibody detects the phosphorylated form of the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) at serine 65. a key regulatory site in cellular translation control. 4E-BP1 inhibits cap-dependent translation by binding to the eukaryotic initiation factor 4E (eIF4E), preventing its interaction with eIF4G to form the eIF4F complex. Phosphorylation of 4E-BP1. particularly at Ser65. disrupts this interaction, releasing eIF4E to promote ribosome recruitment and protein synthesis. This phosphorylation event is primarily mediated by the mechanistic target of rapamycin complex 1 (mTORC1) in response to growth factors, nutrients, or cellular stress, linking it to pathways regulating cell growth, proliferation, and survival.
The Phospho-4E-BP1 (Ser65) antibody is widely used in research to assess mTORC1 activity and downstream signaling in conditions such as cancer, metabolic disorders, and aging. It helps evaluate therapeutic responses to mTOR inhibitors (e.g., rapamycin) or metabolic interventions. Specificity for the phosphorylated Ser65 epitope ensures accurate detection of the active, mTOR-regulated form of 4E-BP1. Applications include Western blotting, immunofluorescence, and immunohistochemistry, often paired with total 4E-BP1 antibodies for normalization. Proper sample preparation (e.g., phosphatase inhibitors) and timing are critical, as phosphorylation is dynamic and context-dependent. This antibody remains a vital tool for studying translational regulation and mTOR pathway dysregulation in disease.
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