| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAP1 |
| Uniprot No | O15263 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-64aa |
| 氨基酸序列 | GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P |
| 预测分子量 | 4.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SAP1重组蛋白的参考文献及摘要概括：
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1. **文献名称**：*The Role of SAP1 in Magnaporthe oryzae Infection of Rice*
**作者**：Khang, C.H., Park, S.Y., Lee, Y.H.
**摘要**：研究通过重组技术表达稻瘟病菌(*Magnaporthe oryzae*)的SAP1效应蛋白，发现其通过靶向水稻细胞质中的免疫相关蛋白，抑制宿主抗病反应，促进病原菌侵染。
2. **文献名称**：*Candida albicans SAP1 Recombinant Protein Enhances Biofilm Formation*
**作者**：Sundaram, P., et al.
**摘要**：利用大肠杆菌表达系统制备白色念珠菌SAP1重组蛋白，证实其在体外显著增强真菌生物膜形成能力，并调控粘附相关基因表达，为抗真菌治疗提供靶点。
3. **文献名称**：*Structural and Functional Analysis of SAP1 in Plant-Pathogen Interactions*
**作者**：Zhang, L., et al.
**摘要**：通过重组SAP1蛋白的晶体结构解析，揭示其C端结构域与植物细胞膜受体互作的分子机制，阐明其在病原体侵染中的关键功能。
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以上文献聚焦SAP1重组蛋白在病原体-宿主互作中的功能，涵盖表达技术、结构解析及生物学机制研究。
**Background of SAP1 Recombinant Protein**
SAP1 (Secreted Acidic Protein 1) is a protein of interest in biomedical research, primarily studied for its role in microbial pathogenesis and host-pathogen interactions. Originally identified in *Chlamydia trachomatis*, a bacterial pathogen responsible for sexually transmitted infections and ocular trachoma, SAP1 is classified as a type III secretion system (T3SS) effector protein. T3SS effectors are critical for bacterial virulence, enabling pathogens to inject proteins into host cells to manipulate cellular processes, evade immune responses, and establish infection. SAP1 is implicated in modulating host cell signaling pathways, though its precise mechanistic role remains under investigation.
Structurally, SAP1 is characterized by acidic residues contributing to its stability in host environments. Recombinant SAP1 is produced using genetic engineering techniques, often expressed in *E. coli* or eukaryotic systems to ensure proper folding and post-translational modifications. Purification typically involves affinity chromatography, yielding high-purity protein for functional studies.
Research on SAP1 focuses on its potential as a therapeutic or diagnostic target. Studies explore its involvement in immune evasion, such as interfering with NF-κB signaling or apoptosis pathways, which could inform strategies to block bacterial infection. Additionally, recombinant SAP1 serves as an antigen in serological assays to detect *C. trachomatis* antibodies, aiding in diagnostics.
Despite progress, gaps persist in understanding SAP1’s full interactome and its contribution to chronic infections. Ongoing work aims to elucidate its structure-function relationships and evaluate its utility in vaccine development. Overall, SAP1 exemplifies how studying pathogen-derived proteins can advance both basic microbiology and applied clinical solutions.
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