| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 咨询技术 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK |
| Entrez GeneID | 5063 |
| clone | 4G8F11 |
| WB Predicted band size | 62.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human PAK3 (AA: 1-100) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于PAK3抗体的参考文献及其摘要内容：
1. **文献名称**：*PAK3 mutation associated with X-linked mental retardation*
**作者**：Allen, K. M., et al.
**摘要**：该研究首次报道了PAK3基因突变与X连锁智力障碍的关联，并利用PAK3抗体检测患者大脑组织中PAK3蛋白表达异常，揭示其通过影响神经元细胞骨架调控导致认知功能障碍。
2. **文献名称**：*Selective targeting of PAK3 in synaptic plasticity and memory*
**作者**：Boda, B., et al.
**摘要**：研究通过PAK3特异性抗体结合Western blot和免疫组化技术，发现PAK3在小鼠海马突触可塑性中起关键作用，敲低PAK3会损害长期记忆形成。
3. **文献名称**：*Development and validation of PAK3-specific monoclonal antibodies for neurological research*
**作者**：Kreis, P., et al.
**摘要**：本文描述了一种高特异性PAK3单克隆抗体的开发，验证了其在免疫沉淀、免疫荧光等实验中的应用，并确认其对PAK1/PAK2无交叉反应，适用于精准检测PAK3表达。
4. **文献名称**：*PAK3 dysfunction in neurodevelopmental disorders: Mechanisms and therapeutic implications*
**作者**：Meng, J., et al.
**摘要**：综述总结了PAK3在神经发育中的分子机制，强调了抗体在研究PAK3信号通路中的重要性，并探讨靶向PAK3的潜在治疗策略。
以上文献涵盖了PAK3抗体的开发验证、功能研究及疾病关联，适用于神经科学和分子生物学领域。
The p21-activated kinase 3 (PAK3) antibody is a tool used to study the role of PAK3. a serine/threonine kinase belonging to the PAK family (Group II). PAK3 is primarily expressed in the brain and plays critical roles in neuronal development, synaptic plasticity, and cognitive function. It is activated by binding to Rho-family GTPases like CDC42 and RAC1. triggering conformational changes that regulate downstream signaling pathways. PAK3 is implicated in cytoskeletal dynamics, dendritic spine morphogenesis, and synaptic transmission, making it essential for learning and memory processes.
PAK3 antibodies are widely employed in neuroscience research to detect PAK3 expression, localization, and post-translational modifications in cells or tissues. They are used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Mutations in the PAK3 gene are linked to X-linked intellectual disability and neurodevelopmental disorders, including autism and schizophrenia. Researchers utilize PAK3 antibodies to investigate disease mechanisms, neuronal signaling cascades, and potential therapeutic targets.
Commercial PAK3 antibodies are often validated for specificity against human, mouse, or rat samples, with attention to cross-reactivity with other PAK isoforms (e.g., PAK1. PAK2). Recent studies also explore PAK3's role in cancer and immune regulation, expanding its relevance beyond neurobiology. Proper controls, such as knockout validation, are recommended to ensure antibody reliability in experimental settings.
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