| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ASH2; Bre2; ASH2L1; ASH2L2 |
| Entrez GeneID | 9070 |
| clone | 6B10H10 |
| WB Predicted band size | 68.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human ASH2L (AA: 493-628) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于ASH2L抗体的3篇参考文献示例(注：文献为虚构示例，实际引用需根据真实研究补充)：
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1. **文献名称**：*ASH2L Antibody Validation in Epigenetic Regulation Studies*
**作者**：Li, X., et al.
**摘要**：本研究开发并验证了一种高特异性ASH2L多克隆抗体，用于检测其在哺乳动物细胞核内的表达。通过Western blot和免疫荧光实验，证实该抗体可特异性识别ASH2L蛋白，并成功应用于染色质免疫沉淀(ChIP)分析，揭示了ASH2L在H3K4三甲基化调控中的关键作用。
2. **文献名称**：*Role of ASH2L in Leukemogenesis via Histone Methylation*
**作者**：Garcia, R., et al.
**摘要**：利用ASH2L单克隆抗体，研究者通过ChIP-seq和CRISPR敲除技术，发现ASH2L在急性髓系白血病(AML)中异常富集于致癌基因启动子区域，促进H3K4me3修饰，驱动肿瘤发生。抗体特异性验证显示其适用于临床样本的免疫组化分析。
3. **文献名称**：*Structural Insights into ASH2L-Containing Methyltransferase Complexes*
**作者**：Wang, Y., et al.
**摘要**：通过免疫共沉淀(Co-IP)结合质谱分析，本研究解析了ASH2L与WRAD复合体(WDR5、RBBP5、Dpy-30)的相互作用。使用ASH2L抗体成功捕获完整复合物，揭示了其在表观遗传调控中的结构基础，为靶向ASH2L的抑制剂设计提供依据。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词“ASH2L antibody”或“ASH2L function”，并筛选涉及抗体应用的研究。
ASH2L (Absent Small and Homeotic disks protein 2 homolog) is a core subunit of the multi-protein COMPASS (Complex of Proteins Associated with Set1) family, which plays a critical role in epigenetic regulation by mediating histone H3 lysine 4 methylation (H3K4me). This post-translational modification is associated with active gene transcription. ASH2L stabilizes the COMPASS complex and enhances its methyltransferase activity, particularly for di- and tri-methylation (H3K4me2/me3), which are hallmarks of promoter and enhancer regions in actively transcribed genes.
ASH2L antibodies are widely used tools to study its function in development, differentiation, and disease. They enable researchers to detect ASH2L protein expression via Western blotting (WB), visualize its nuclear localization through immunofluorescence (IF), or identify its binding sites genome-wide using chromatin immunoprecipitation (ChIP-seq). Dysregulation of ASH2L has been linked to cancers, developmental disorders, and metabolic diseases, as it influences cell proliferation and differentiation pathways. Antibodies against ASH2L also help investigate its interactions with other COMPASS components (e.g., WDR5. RBBP5) and explore therapeutic strategies targeting epigenetic regulators. Due to its conserved role in maintaining transcriptional fidelity, ASH2L remains a key focus in epigenetics and molecular biology research.
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