Mouse Monoclonal CBX2 Antibody

The CBX2 antibody is a crucial tool for studying the Chromobox homolog 2 (CBX2) protein, a member of the Polycomb Repressive Complex 1 (PRC1) family involved in epigenetic regulation. CBX2 plays a key role in maintaining transcriptional repression through chromatin modification, such as facilitating H2A ubiquitination, and contributes to cell differentiation, development, and stem cell pluripotency. It is particularly noted for its role in sexual differentiation and organogenesis. Antibodies targeting CBX2 are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and interaction with chromatin. These antibodies are essential in research on cancers (e.g., prostate, breast), developmental disorders, and metabolic diseases, where dysregulation of CBX2 is linked to pathological processes. Most CBX2 antibodies are raised against specific epitopes, often within conserved regions like the chromodomain or C-terminal segments. Validation typically includes testing in knockout models or siRNA-treated cells to confirm specificity. Commercial antibodies vary by clonality (monoclonal/polyoclonal), host species (rabbit, mouse), and conjugates (e.g., HRP, FITC). Researchers must consider cross-reactivity with other CBX family members (e.g., CBX4. CBX8) and verify target isoforms, as CBX2 has splice variants. Its study advances understanding of PRC1-mediated gene silencing mechanisms and their therapeutic implications.