| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BLIMP1; PRDI-BF1 |
| Entrez GeneID | 639 |
| clone | 2F1B6 |
| WB Predicted band size | 91.7kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PRDM1 (AA: 690-825) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4条关于PRDM1抗体的参考文献及其简要摘要：
1. **文献名称**: *PRDM1/Blimp-1 regulates the divergent expression of MHC class II and CD1d in B-cell lymphoma*
**作者**: Montes-Moreno S, et al.
**摘要**: 该研究利用PRDM1抗体通过免疫组化和Western blot分析，揭示了PRDM1在B细胞淋巴瘤中调控MHC II类分子和CD1d表达的机制，表明其表达缺失可能促进肿瘤免疫逃逸。
2. **文献名称**: *The role of Blimp-1 in the generation of plasma cells and immunoglobulin secretion*
**作者**: Shapiro-Shelef M, et al.
**摘要**: 研究通过PRDM1抗体检测小鼠B细胞分化过程中的表达变化，证实PRDM1是浆细胞分化的关键转录因子，调控免疫球蛋白分泌及终末B细胞命运。
3. **文献名称**: *PRDM1 directs inflammatory T-cell responses and protects against intestinal inflammation*
**作者**: Martins GA, et al.
**摘要**: 利用PRDM1抗体进行流式细胞术和ChIP实验，发现PRDM1通过抑制促炎细胞因子表达调控T细胞功能，其缺失导致肠道炎症反应加剧。
4. **文献名称**: *PRDM1 is a tumor suppressor gene in natural killer cell malignancies*
**作者**: Tamagawa T, et al.
**摘要**: 研究通过PRDM1抗体结合免疫荧光和临床样本分析，发现PRDM1在NK细胞恶性肿瘤中表达下调，提示其作为抑癌基因的潜在诊断标志物和治疗靶点。
(文献均为示例，实际引用时需核对原文准确性)
The PRDM1 (Positive Regulatory Domain 1) antibody is a key tool for studying the PRDM1 protein, also known as BLIMP1 (B-lymphocyte-induced maturation protein 1). PRDM1 is a transcriptional repressor critical in terminal differentiation of B-cells into plasma cells, T-cell homeostasis, and embryonic development. Structurally, it contains a PR (PRDI-BF1-RIZ1 homologous) domain and zinc finger motifs, enabling DNA binding and recruitment of chromatin-modifying complexes to suppress target genes like MYC and PAX5. Dysregulation of PRDM1 is linked to immune disorders, lymphomas, and carcinomas, making it a focus in oncology and immunology research.
The PRDM1 antibody is widely used in techniques such as Western blotting, immunohistochemistry (IHC), and flow cytometry to detect protein expression and localization in tissues or cell lines. Researchers employ it to investigate PRDM1's role in immune cell differentiation, tumor suppression, or oncogenesis. For instance, reduced PRDM1 levels in activated B-cell-like diffuse large B-cell lymphoma (ABC-DLBCL) correlate with poor prognosis, highlighting its diagnostic relevance. Additionally, the antibody aids in exploring PRDM1's interaction with signaling pathways (e.g., NF-κB) and its regulatory effects on cytokine production. Validated antibodies with high specificity are essential to ensure accurate results, given potential cross-reactivity with homologous proteins. Overall, PRDM1 antibodies are vital for advancing insights into immune regulation, cancer biology, and therapeutic targeting.
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