| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | M33; CDCA6; SRXY5 |
| Entrez GeneID | 84733 |
| clone | 4D10B2 |
| WB Predicted band size | 56kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human CBX2 (AA: 402-525) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于CBX2抗体的参考文献摘要概览(文献标题为虚构示例，仅用于说明格式)：
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1. **文献名称**: *CBX2 regulates human ovarian development through histone modification*
**作者**: Smith A, et al.
**摘要**: 本研究使用CBX2特异性抗体进行免疫组化分析，发现CBX2在人类胎儿性腺发育中高表达，并通过调控H3K27me3修饰参与性别决定通路。抗体验证实验显示其在卵巢组织中的特异性定位。
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2. **文献名称**: *CBX2 as a biomarker in prostate cancer progression*
**作者**: Lee JH, et al.
**摘要**: 通过Western blot和免疫荧光(使用兔源CBX2单克隆抗体)，研究发现CBX2在前列腺癌转移样本中显著上调，其表达水平与患者生存率负相关，提示其可作为潜在治疗靶点。
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3. **文献名称**: *Chromatin targeting of CBX2 in pluripotent stem cells*
**作者**: García-Pérez R, et al.
**摘要**: 利用ChIP-seq技术(基于CBX2抗体的染色质免疫沉淀)揭示CBX2通过结合多梳抑制复合物(PRC1)调控胚胎干细胞的自我更新，抗体特异性通过siRNA敲除实验验证。
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注：以上文献为虚构示例，实际文献需通过PubMed/Google Scholar以关键词"CBX2 antibody"+"应用领域(如cancer, development)"检索真实数据。
The CBX2 antibody targets the Chromobox homolog 2 (CBX2) protein, a key component of the Polycomb Repressive Complex 1 (PRC1) involved in epigenetic gene regulation. CBX2 facilitates PRC1-mediated transcriptional repression by binding to trimethylated lysine 27 on histone H3 (H3K27me3), a mark deposited by Polycomb Repressive Complex 2 (PRC2). This interaction enables chromatin compaction and maintenance of silenced gene states, playing critical roles in cellular differentiation, development, and stem cell pluripotency.
CBX2 is particularly noted for its function in sex determination and gonadal development, as mutations or dysregulation are linked to 46.XY disorders of sexual development. It also contributes to cancer progression, with overexpression observed in prostate, ovarian, and other cancers, where it promotes proliferation, metastasis, and therapy resistance by silencing tumor suppressors.
Antibodies against CBX2 are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunofluorescence, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP). Researchers rely on these antibodies to investigate CBX2's role in epigenetic mechanisms, developmental biology, and oncogenesis. Specificity and validation (e.g., knockout controls) are critical, as CBX2 shares homology with other Polycomb family members (CBX4. CBX6-8). Commercial antibodies often target unique epitopes to distinguish CBX2 isoforms or post-translational modifications, enabling precise analysis in diverse experimental contexts.
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