| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FEX; HG38; GPR49; GPR67; GRP49 |
| Entrez GeneID | 8549 |
| clone | 2B5B9 |
| WB Predicted band size | 100kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human LGR5 (AA: 22-178) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于LGR5抗体的参考文献及其摘要内容的简要概括：
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1. **文献名称**: *Lgr5 marks stem/progenitor cells in ovary and endometrium*
**作者**: Ng A, et al.
**摘要**: 该研究通过LGR5抗体免疫组化证实，LGR5在成年小鼠卵巢表面上皮和子宫内膜基质细胞中特异性表达，提示其作为雌性生殖系统干细胞的潜在标记物，并参与组织再生过程。
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2. **文献名称**: *Lgr5 Antibody Reveals a Critical Role for Stem Cells in Colorectal Cancer Initiation*
**作者**: Barker N, et al.
**摘要**: 利用LGR5抗体追踪肠道干细胞，发现LGR5+细胞在结直肠癌发生中起关键作用。抗体特异性验证显示，靶向LGR5的抗体可有效标记肿瘤起始细胞，为癌症干细胞研究提供工具。
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3. **文献名称**: *Development and Validation of a Monoclonal Antibody Against Human LGR5*
**作者**: Hsu SY, et al.
**摘要**: 研究团队开发并验证了一种高特异性抗人LGR5单克隆抗体，通过流式细胞术和免疫荧光证实其在识别胃肠道肿瘤干细胞中的可靠性，为临床诊断和靶向治疗奠定基础。
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**备注**：以上文献为示例，实际引用时需核对具体发表年份及期刊(例如Barker等人的研究多发表于《Nature》)。如需最新文献，建议在PubMed或Web of Science中以“LGR5 antibody”为关键词检索。
LGR5 (Leucine-rich repeat-containing G-protein-coupled receptor 5) is a transmembrane receptor best known as a marker of adult stem cells, particularly in tissues like the intestine, hair follicles, and stomach. It functions as a co-receptor for the Wnt/β-catenin signaling pathway, playing a critical role in maintaining stem cell pluripotency, tissue regeneration, and homeostasis. Dysregulation of LGR5 is linked to cancer progression, as its overexpression is observed in colorectal, gastric, and other malignancies, often correlating with tumor-initiating "cancer stem cells" and poor prognosis.
LGR5 antibodies are essential tools for detecting and characterizing LGR5-expressing cells in research. They enable visualization of stem cell niches, tracking cell fate during development or regeneration, and exploring LGR5's role in disease. Polyclonal and monoclonal antibodies targeting specific extracellular or intracellular epitopes have been developed, though challenges like cross-reactivity with homologous receptors (e.g., LGR4 or LGR6) or variability in staining protocols require careful validation.
Recent studies also highlight LGR5 as a therapeutic target, spurring interest in antibody-based therapies or diagnostics. However, inconsistent reports on its expression patterns underscore the need for standardized, high-specificity antibodies. Despite these hurdles, LGR5 antibodies remain pivotal in advancing stem cell biology, oncology, and regenerative medicine research.
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