| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A |
| Entrez GeneID | 9759 |
| clone | 7E2E6 |
| WB Predicted band size | 119kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HDAC4 (AA: 456-592) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于HDAC4抗体的典型文献(虚构示例，仅供参考实际文献需查询数据库)：
1. **文献名称**：*"HDAC4 regulates neuronal survival via calcium/calmodulin-dependent signaling"*
**作者**：Miska EA et al.
**摘要**：研究利用特异性HDAC4抗体检测其在神经元中的亚细胞定位，发现其核质穿梭与钙信号调控神经元凋亡相关，抗体验证通过Western blot和免疫荧光。
2. **文献名称**：*"Phosphorylation-dependent interaction of HDAC4 with 14-3-3 proteins"*
**作者**：Wang AH et al.
**摘要**：通过磷酸化特异性HDAC4抗体，揭示其丝氨酸磷酸化修饰促进与14-3-3蛋白结合，进而影响HDAC4的核输出，抗体特异性经点突变实验验证。
3. **文献名称**：*"Antibody characterization of HDAC4 in cardiac hypertrophy models"*
**作者**：Backs J et al.
**摘要**：开发并验证一种高灵敏度HDAC4抗体，用于心肌肥厚小鼠模型的免疫组化分析，证明HDAC4在病理心脏组织中的表达下调与染色质重塑相关。
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**实际文献建议查询**：
- PubMed/Google Scholar中检索“HDAC4 antibody validation”或“HDAC4 immunohistochemistry”等关键词，筛选涉及抗体应用及验证的论文。
- 可参考抗体公司(如CST、Abcam)官网提供的文献引用，通常列出已发表研究中该抗体的使用案例。
HDAC4 (Histone Deacetylase 4) is a member of the class IIa HDAC family, which plays a critical role in regulating gene expression by modifying chromatin structure through the removal of acetyl groups from histone proteins. This enzyme is involved in diverse cellular processes, including differentiation, apoptosis, and stress response, with tissue-specific roles in muscle, bone, and brain development. HDAC4 primarily shuttles between the nucleus and cytoplasm, interacting with transcription factors like MEF2 to repress target genes. Dysregulation of HDAC4 has been linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.
HDAC4 antibodies are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to detect HDAC4 protein levels, subcellular distribution, and protein-protein interactions. Specificity and validation are critical, as cross-reactivity with other HDAC family members (e.g., HDAC5. HDAC7) can occur. High-quality HDAC4 antibodies are often validated using knockout cell lines or tissues to ensure reliability. Research applications include exploring HDAC4's role in epigenetic regulation, its phosphorylation-dependent nucleocytoplasmic trafficking, and its potential as a therapeutic target, particularly in diseases involving aberrant histone acetylation patterns.
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