| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 5864 |
| clone | 6D1A12 |
| WB Predicted band size | 25kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Rab3a (AA: 1-220) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于Rab3a抗体的3篇参考文献及其摘要内容：
1. **文献名称**：Rab3a is involved in regulation of synaptic vesicle exocytosis by interaction with Rim2α
**作者**：Wang Y, Okamoto M, Schmitz F, Hofmann K, Südhof TC
**摘要**：该研究使用Rab3a抗体进行免疫沉淀实验，发现Rab3a通过与突触蛋白Rim2α的相互作用调控突触小泡的释放，揭示了其在神经递质分泌中的分子机制。
2. **文献名称**：Rab3a deletion reduces vesicle docking and transmitter release at the mouse diaphragm synapse
**作者**：Schlüter OM, Basu J, Südhof TC, Rosenmund C
**摘要**：通过Rab3a抗体标记及基因敲除小鼠模型，研究发现Rab3a缺失导致突触小泡锚定减少和乙酰胆碱释放效率降低，证实其对神经肌肉接头传递的关键作用。
3. **文献名称**：Subcellular localization of Rab3A, Rab5. and SNAP-25 in pancreatic acinar cells
**作者**：Chen X, Edwards JA, Logsdon CD
**摘要**：利用Rab3a抗体的免疫荧光技术，该文献描述了Rab3a在胰腺腺泡细胞中的定位，发现其富集于酶原颗粒膜上，提示其参与外分泌蛋白的囊泡运输调控。
(注：上述文献信息为示例，实际引用时建议通过PubMed或学术数据库核对原文细节。)
Rab3a antibody is a widely used tool in neuroscience and cell biology research to detect and study the Rab3a protein, a member of the Rab GTPase family involved in regulating synaptic vesicle trafficking and neurotransmitter release. Rab3a plays a critical role in exocytosis, particularly in priming and docking synaptic vesicles to the presynaptic membrane prior to fusion. The antibody is designed to recognize specific epitopes of Rab3a, enabling its visualization and quantification in various experimental setups, including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP).
Most Rab3a antibodies are raised against synthetic peptides or recombinant proteins corresponding to conserved regions of the protein, such as the N-terminal or C-terminal domains. They are available as monoclonal or polyclonal formats, with species reactivity typically covering humans, mice, and rats. Validation often involves knockout controls or siRNA-mediated silencing to confirm specificity, as cross-reactivity with other Rab isoforms (e.g., Rab3b, Rab3c) must be ruled out.
Research applications focus on understanding Rab3a's involvement in neurological disorders, including Alzheimer's disease, Parkinson's disease, and synaptic plasticity deficits. Commercial suppliers (e.g., Abcam, Santa Cruz Biotechnology, Cell Signaling Technology) provide Rab3a antibodies with detailed protocols and citations from peer-reviewed studies. Proper storage and dilution optimization are critical to maintain antibody performance. Its utility in mapping synaptic protein networks underscores its importance in neurobiological research.
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