首页 / 产品 / 抗体 / 一抗

Mouse Monoclonal PHC1 Antibody

  • 中文名: PHC1抗体
  • 别    名: EDR1; HPH1; RAE28
货号: IPD30499
Price: ¥1280
数量:
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/200 - 1/400 Human,Mouse,Rat
Elisa 1/10000 Human,Mouse,Rat

产品详情

AliasesEDR1; HPH1; RAE28
Entrez GeneID1911
clone1F3F3
WB Predicted band size105.5kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant fragment of human PHC1 (AA: 758-1004) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

+ +

参考文献

以下是关于PHC1抗体的3篇参考文献及其摘要概括:

1. **文献名称**:*"Polycomb group proteins mediate histone H3K27 methylation to regulate gene silencing in Drosophila"*

**作者**:Cao, R., Wang, L., Wang, H., Xia, L., Erdjument-Bromage, H., Tempst, P., et al.

**摘要**:该研究利用PHC1抗体通过免疫沉淀和染色质分析,揭示了PHC1作为PRC1复合体的核心组分,在果蝇中通过介导H2A泛素化参与基因沉默和表观遗传调控的机制。

2. **文献名称**:*"Role of PHC1 in maintaining pluripotency of embryonic stem cells through PRC1 complex activity"*

**作者**:Endoh, M., Endo, T.A., Shinga, J., Sharif, J., Ohara, O., & Vidal, M.

**摘要**:通过PHC1抗体的免疫荧光和ChIP-seq实验,研究发现PHC1通过PRC1复合体维持胚胎干细胞的自我更新能力,调控多能性相关基因的H2A泛素化修饰和染色质压缩。

3. **文献名称**:*"PHC1 interacts with BMI1 and is essential for the DNA damage response in mammalian cells"*

**作者**:Li, X., Zhang, Y., Zheng, L., Liu, M., Chen, C.D., & Jiang, H.

**摘要**:该文献使用PHC1抗体进行Western blot和共免疫沉淀实验,证实PHC1与BMI1相互作用,在DNA损伤修复中通过调控PRC1复合体的招募维持基因组稳定性。

以上文献均通过PHC1抗体探究了其在基因沉默、干细胞多能性和DNA损伤应答中的功能,可作为相关研究的参考。

背景信息

The PHC1 (Polyhomeotic Homolog 1) antibody is a tool used to study the PHC1 protein, a core component of the Polycomb Repressive Complex 1 (PRC1) within the Polycomb group (PcG) family. PcG proteins are evolutionarily conserved epigenetic regulators that maintain transcriptional repression of genes, particularly those involved in cell fate determination, differentiation, and development. PHC1. along with other PRC1 subunits (e.g., BMI1. RING1B), facilitates chromatin compaction and monoubiquitination of histone H2A at lysine 119 (H2AK119ub), reinforcing gene silencing. This activity is critical for preserving stem cell pluripotency and ensuring proper embryonic development. Dysregulation of PHC1/PRC1 is linked to cancers, neurological disorders, and developmental anomalies. PHC1 antibodies are widely employed in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and interactions in cellular and disease models. They also aid in exploring PRC1's role in epigenetic memory, X-chromosome inactivation, and tumorigenesis. Validating PHC1 antibody specificity is essential due to structural similarities among PcG proteins. Research utilizing these antibodies has advanced understanding of Polycomb-mediated gene regulation and its therapeutic implications.

客户数据及评论

折叠内容

大包装询价

×