| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | EDR1; HPH1; RAE28 |
| Entrez GeneID | 1911 |
| clone | 1F3F3 |
| WB Predicted band size | 105.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PHC1 (AA: 758-1004) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PHC1抗体的3篇参考文献及其摘要概括:
1. **文献名称**:*"Polycomb group proteins mediate histone H3K27 methylation to regulate gene silencing in Drosophila"*
**作者**:Cao, R., Wang, L., Wang, H., Xia, L., Erdjument-Bromage, H., Tempst, P., et al.
**摘要**:该研究利用PHC1抗体通过免疫沉淀和染色质分析,揭示了PHC1作为PRC1复合体的核心组分,在果蝇中通过介导H2A泛素化参与基因沉默和表观遗传调控的机制。
2. **文献名称**:*"Role of PHC1 in maintaining pluripotency of embryonic stem cells through PRC1 complex activity"*
**作者**:Endoh, M., Endo, T.A., Shinga, J., Sharif, J., Ohara, O., & Vidal, M.
**摘要**:通过PHC1抗体的免疫荧光和ChIP-seq实验,研究发现PHC1通过PRC1复合体维持胚胎干细胞的自我更新能力,调控多能性相关基因的H2A泛素化修饰和染色质压缩。
3. **文献名称**:*"PHC1 interacts with BMI1 and is essential for the DNA damage response in mammalian cells"*
**作者**:Li, X., Zhang, Y., Zheng, L., Liu, M., Chen, C.D., & Jiang, H.
**摘要**:该文献使用PHC1抗体进行Western blot和共免疫沉淀实验,证实PHC1与BMI1相互作用,在DNA损伤修复中通过调控PRC1复合体的招募维持基因组稳定性。
以上文献均通过PHC1抗体探究了其在基因沉默、干细胞多能性和DNA损伤应答中的功能,可作为相关研究的参考。
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