| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GATA6 |
| Entrez GeneID | 2627 |
| clone | 2F10G3 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human GATA6 (AA: 491-557) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与GATA6抗体相关的参考文献概览(基于公开信息模拟整理，非真实文献)：
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1. **标题**：*GATA6 is required for proliferation and differentiation of committed epithelial progenitors in the developing pancreas*
**作者**：Xuan, S., et al.
**摘要**：本研究利用GATA6特异性抗体(来源：小鼠单克隆，货号AB123)通过免疫荧光和Western blot分析，揭示了GATA6在胰腺胚胎发育中对上皮前体细胞增殖和分化的调控作用，证实抗体在组织染色中的高特异性。
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2. **标题**：*GATA6 expression distinguishes classical and basal subtypes in pancreatic ductal adenocarcinoma*
**作者**：Martinelli, P., et al.
**摘要**：通过免疫组化(兔多克隆抗体，货号sc-1234)对胰腺癌亚型进行分型，发现GATA6高表达与经典亚型相关，且抗体染色结果与患者预后显著相关，验证了其在临床病理诊断中的潜在价值。
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3. **标题**：*Validation of commercial GATA6 antibodies for reproducible detection in human and mouse tissues*
**作者**：Zhang, L., et al.
**摘要**：系统性比较5种市售GATA6抗体(包括ABCam#xyz和CST#abc)的灵敏度与特异性，通过敲除细胞系和多种组织验证，推荐特定抗体适用于不同实验场景(如ChIP-seq或冰冻切片染色)。
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**备注**：以上为模拟示例，实际文献需通过PubMed或Google Scholar检索关键词“GATA6 antibody + application”(如特定疾病或技术方法)获取。如需具体文献，建议访问学术数据库查询。
The GATA6 antibody is a crucial tool in studying the GATA6 protein, a member of the GATA transcription factor family characterized by zinc finger DNA-binding domains. GATA6 plays a pivotal role in embryonic development, particularly in endoderm-derived organs (e.g., gastrointestinal tract, lungs, pancreas), regulating cell differentiation, proliferation, and tissue morphogenesis. It is also implicated in adult tissue homeostasis and disease processes, including cancer, cardiovascular disorders, and diabetes.
As a research reagent, GATA6 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and interactions. They help identify GATA6's regulatory roles in gene networks, its crosstalk with signaling pathways (e.g., Wnt/β-catenin), and its dual oncogenic/tumor-suppressive functions depending on tissue context. Commercially available GATA6 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal regions). Validation includes testing on knockout controls and relevant cell lines (e.g., pancreatic or colorectal cancer models).
Dysregulation of GATA6 is linked to developmental anomalies and diseases, making its antibody essential for mechanistic studies and potential therapeutic targeting. Researchers rely on its specificity to explore tissue-specific gene regulation and disease biomarkers.
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