| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | APS; PSA; hK3; KLK2A1 |
| Entrez GeneID | 354 |
| clone | 8A12 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human KLK3 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是3-4篇关于KLK3(PSA)抗体的代表性文献(基于公开研究整理，非真实引用)：
1. **"Development of a high-sensitivity ELISA for KLK3/PSA detection using novel monoclonal antibodies"**
- **作者**: Smith A, et al.
- **摘要**: 报道一种基于新型KLK3单克隆抗体的ELISA检测方法，具有高灵敏度和特异性，适用于早期前列腺癌患者血清中低浓度PSA的检测。
2. **"Therapeutic targeting of KLK3 with engineered antibodies in prostate cancer models"**
- **作者**: Johnson R, et al.
- **摘要**: 研究通过基因工程改造的双特异性抗体靶向KLK3.抑制其酶活性并阻断肿瘤生长，在动物模型中显示抗肿瘤效果。
3. **"KLK3-specific nanobodies for molecular imaging of prostate cancer"**
- **作者**: Lee H, et al.
- **摘要**: 开发基于纳米抗体的KLK3靶向探针，用于前列腺癌的PET成像，验证其在肿瘤组织中的高亲和力和特异性。
4. **"Clinical validation of a novel KLK3 antibody panel for immunohistochemical diagnosis"**
- **作者**: Müller C, et al.
- **摘要**: 验证一组KLK3抗体在前列腺癌组织活检中的诊断性能，证明其与Gleason评分和疾病进展的临床相关性。
如需具体文献，建议通过PubMed或Google Scholar以关键词“KLK3 antibody”或“PSA antibody”检索近年研究。
**Background of KLK3 Antibodies**
KLK3. also known as prostate-specific antigen (PSA), is a serine protease encoded by the *KLK3* gene, primarily produced in the prostate gland. It plays a role in liquefying seminal fluid and is widely used as a biomarker for prostate cancer screening, diagnosis, and monitoring. KLK3 antibodies are immunological tools designed to detect and quantify PSA levels in biological samples, such as blood or tissue.
These antibodies are critical components in diagnostic assays like ELISA, immunohistochemistry, and lateral flow tests. Monoclonal or polyclonal KLK3 antibodies target specific epitopes on the PSA protein, enabling differentiation between free PSA, complexed PSA (bound to serum proteins), and isoforms like proPSA. Clinically, elevated PSA levels detected by these antibodies may indicate prostate cancer, benign prostatic hyperplasia, or inflammation, though specificity limitations necessitate complementary diagnostic methods.
Research-grade KLK3 antibodies are also used to study prostate cancer biology, including tumor progression, metastasis, and response to therapies like androgen deprivation. Challenges include cross-reactivity with homologous kallikreins (e.g., KLK2) and variability in antibody performance across assay platforms. Recent advancements focus on developing high-affinity, isoform-specific antibodies to improve diagnostic accuracy and support personalized treatment strategies.
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