| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | NKX2B;NKX2-2 |
| Entrez GeneID | 4821 |
| clone | 3E4 |
| WB Predicted band size | 30.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NKX2.2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NKX2.2抗体的3篇示例文献(注：内容为示例性概括，具体文献需根据实际数据库检索验证)：
1. **文献名称**：*NKX2.2 as a novel immunohistochemical marker for the diagnosis of Ewing sarcoma*
**作者**：Suster, D. et al.
**摘要**：研究验证了NKX2.2抗体在尤文肉瘤中的特异性表达，证明其在区分尤文肉瘤与其他小圆细胞肿瘤中的诊断价值，支持其作为辅助病理标记物的临床应用。
2. **文献名称**：*The role of NKX2.2 in pancreatic β-cell development and function*
**作者**：Hung, Y.H. et al.
**摘要**：探讨了NKX2.2抗体在胰岛β细胞发育研究中的应用，通过免疫组化和基因敲除模型揭示了该转录因子对胰岛素分泌调控的关键作用。
3. **文献名称**：*Comparative analysis of NKX2.2 and CD99 in small round blue cell tumors*
**作者**：Rudelius, M. et al.
**摘要**：对比NKX2.2抗体与CD99在儿童小圆细胞肿瘤中的敏感性和特异性，强调NKX2.2在尤文肉瘤和神经外胚层肿瘤中的互补诊断意义。
建议通过PubMed或Google Scholar检索具体文献以获取详细信息。
NKX2.2 antibody is a valuable tool in diagnostic pathology and research, primarily targeting the NKX2.2 protein encoded by the *NKX2-2* gene. This transcription factor belongs to the NKX2 family of homeodomain-containing proteins, which play critical roles in embryonic development, particularly in the differentiation of the central nervous system, pancreas, and lungs. NKX2.2 is essential for specifying neuronal and endocrine cell lineages, with its expression tightly regulated during neurogenesis and pancreatic β-cell development.
In diagnostic contexts, NKX2.2 antibody is widely used to identify tumors of neural and neuroendocrine origin. It is a sensitive marker for Ewing sarcoma, a malignant bone/soft tissue tumor characterized by *EWSR1* gene rearrangements. NKX2.2 immunohistochemical positivity helps differentiate Ewing sarcoma from histologic mimics like small round blue cell tumors (e.g., lymphomas or rhabdomyosarcoma). It also aids in diagnosing other neuroendocrine neoplasms, such as pancreatic neuroendocrine tumors and olfactory neuroblastoma. However, specificity varies, and results must be interpreted alongside clinical, morphologic, and molecular data.
Research applications include studying developmental biology, neuroendocrine differentiation pathways, and tumorigenesis mechanisms. Commercial NKX2.2 antibodies are typically monoclonal, validated for immunohistochemistry (IHC) and/or immunofluorescence (IF), with reactivity across human and murine tissues.
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