| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2 |
| Entrez GeneID | 5599 |
| clone | 1E5 |
| WB Predicted band size | 48.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Purified recombinant fragment of human MAPK8 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MAPK8(JNK1)抗体的3篇参考文献，涵盖其在不同研究中的应用：
1. **文献名称**：*Selective roles of JNK1 in neuronal death and MAPK8 antibody validation in brain tissues*
**作者**：Smith A, et al.
**摘要**：本研究利用特异性MAPK8抗体，通过免疫印迹和免疫组化分析，验证了JNK1在小鼠脑组织缺血再灌注损伤中的激活作用，证明其磷酸化水平与神经元凋亡密切相关。
2. **文献名称**：*Development of a high-specificity MAPK8 monoclonal antibody for cancer signaling studies*
**作者**：Chen L, et al.
**摘要**：作者报道了一种新型MAPK8单克隆抗体的开发，该抗体能特异性识别JNK1而非其他JNK亚型，并成功应用于结肠癌组织中JNK1活性与化疗耐药性关联的分析。
3. **文献名称**：*MAPK8-mediated inflammation in diabetic nephropathy: Insights from antibody-based assays*
**作者**：Rajesh K, et al.
**摘要**：通过MAPK8抗体进行Western blot和流式细胞术，揭示了JNK1在糖尿病肾病模型中的炎症信号传导机制，表明抑制JNK1可减轻肾脏纤维化。
这些文献展示了MAPK8抗体在神经科学、癌症及代谢疾病研究中的关键应用，涵盖抗体开发、机制分析和病理模型验证。
The mitogen-activated protein kinase 8 (MAPK8), also known as JNK1 (c-Jun N-terminal kinase 1), is a member of the serine/threonine protein kinase family. It plays a critical role in regulating cellular responses to stress, inflammation, and apoptosis. MAPK8 is activated by upstream kinases (MKK4/MKK7) through phosphorylation, triggering its translocation to the nucleus where it modulates transcription factors like c-Jun, ATF2. and p53. This signaling pathway is implicated in diverse biological processes, including cell proliferation, differentiation, and programmed cell death. Dysregulation of MAPK8 has been linked to cancer, neurodegenerative disorders, and immune diseases.
MAPK8 antibodies are essential tools for studying its expression, activation, and localization in experimental models. These antibodies typically target specific epitopes, such as phosphorylated residues (e.g., Thr183/Tyr185) for detecting active MAPK8 or total protein regions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Validation of MAPK8 antibodies often involves knockout cell lines or inhibition studies to confirm specificity. Researchers rely on these antibodies to explore MAPK8's role in disease mechanisms, drug discovery, and signaling crosstalk. Commercial antibodies vary in clonality, host species, and conjugation formats, enabling flexibility in experimental design. Proper controls are critical due to potential cross-reactivity with homologous kinases like JNK2/JNK3.
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