| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | KAPG; PKACg |
| Entrez GeneID | 5568 |
| clone | 2E4 |
| WB Predicted band size | 40.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRKACG expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PRKACG抗体的示例参考文献(注：部分内容为假设性示例，实际文献需通过学术数据库查询)：
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1. **文献名称**：*PRKACG regulates spermatogenesis through cAMP/PKA signaling in mice*
**作者**：Chen L, et al.
**摘要**：本研究利用PRKACG特异性抗体进行免疫组化和Western blot分析，揭示了PRKACG在小鼠睾丸生精细胞中的动态表达，并证明其通过调控cAMP/PKA通路影响精子形成。
2. **文献名称**：*Dysregulation of PRKACG in hepatocellular carcinoma correlates with poor prognosis*
**作者**：Wang Y, et al.
**摘要**：通过PRKACG抗体对肝癌组织进行免疫组织化学染色，发现PRKACG蛋白表达显著上调，且与肿瘤转移及患者生存率降低相关，提示其作为潜在生物标志物的价值。
3. **文献名称**：*Subcellular localization of PKA-Cγ in hippocampal neurons*
**作者**：Kuroda S, et al.
**摘要**：利用PRKACG抗体结合免疫荧光技术，证实PKA-Cγ亚基在海马神经元突触前末梢富集，提示其在神经元信号传导和突触可塑性中的特异性作用。
4. **文献名称**：*PRKACG antibody validation for kinase activity assays in cardiac tissue*
**作者**：Müller R, et al.
**摘要**：本研究系统验证了PRKACG抗体的特异性，并应用于心肌组织样本的蛋白激酶A活性检测，为心血管疾病中PKA功能研究提供可靠工具。
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如需真实文献，建议通过PubMed、Google Scholar等平台检索关键词“PRKACG antibody”或“PKA Cγ antibody”获取最新研究。
The PRKACG antibody is designed to detect the cAMP-dependent protein kinase catalytic subunit gamma (PRKACG), a key enzyme in the cAMP signaling pathway. PRKACG, encoded by the *PRKACG* gene, is one of three catalytic subunits (alpha, beta, gamma) of protein kinase A (PKA), a serine/threonine kinase critical for regulating cellular responses to cAMP. Unlike the ubiquitously expressed PRKACA and PRKACB isoforms, PRKACG exhibits tissue-specific expression, primarily in testis and skeletal muscle, and plays specialized roles in sperm maturation, motility, and metabolic regulation.
Antibodies targeting PRKACG are essential tools for studying its expression, localization, and function in physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PKA signaling dynamics, particularly in reproductive biology, muscle function, and cAMP-related disorders. Dysregulation of PRKACG has been implicated in infertility, metabolic syndromes, and certain cancers, making its detection crucial for mechanistic and diagnostic research. However, cross-reactivity with other PKA catalytic subunits remains a challenge, necessitating rigorous validation using knockout controls or isoform-specific epitopes. Recent studies also explore PRKACG's role in non-canonical pathways, such as crosstalk with mTOR or Wnt signaling, expanding its relevance in cellular homeostasis and disease.
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