| WB | 1/500 - 1/2000 | Human,Mouse,Rat,,Monkey |
| IF | 咨询技术 | Human,Mouse,Rat,,Monkey |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat,,Monkey |
| ICC | 技术咨询 | Human,Mouse,Rat,,Monkey |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat,,Monkey |
| Elisa | 1/10000 | Human,Mouse,Rat,,Monkey |
| Aliases | IPD1; REN64; NY-REN-64 |
| Entrez GeneID | 51135 |
| clone | 2H9 |
| WB Predicted band size | 52kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,,Monkey |
| Immunogen | Purified recombinant fragment of human IRAK4 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与IRAK4抗体相关的虚构参考文献示例(内容基于领域常识构建，非真实文献)：
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1. **标题**: *Structural insights into IRAK4 kinase activation by monoclonal antibody targeting*
**作者**: Chen L, et al.
**摘要**: 本研究通过X射线晶体学解析了IRAK4激酶结构域与特异性单克隆抗体的复合物结构，揭示了抗体结合如何阻断IRAK4的ATP结合口袋，为开发靶向IRAK4的抑制剂提供结构基础。
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2. **标题**: *IRAK4 antibody-based detection of inflammatory signaling in sepsis models*
**作者**: Gupta R, et al.
**摘要**: 开发了一种高特异性IRAK4多克隆抗体，用于检测脓毒症小鼠模型中IRAK4蛋白表达及磷酸化水平变化，证实IRAK4在TLR4/NF-κB通路中的关键调控作用。
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3. **标题**: *A neutralizing IRAK4 antibody attenuates autoimmune arthritis in vivo*
**作者**: Sato K, et al.
**摘要**: 报道了一种中和性IRAK4单抗，可通过抑制MyD88-IRAK4相互作用显著降低胶原诱导性关节炎模型中的促炎细胞因子水平，提示其治疗自身免疫疾病的潜力。
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注：以上为模拟文献，实际研究中建议通过PubMed/Google Scholar以“IRAK4 antibody”、“IRAK4 inhibitor”等关键词检索真实文献。
Interleukin-1 receptor-associated kinase 4 (IRAK4) is a critical serine/threonine kinase in the Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways, which mediate innate immune responses. As part of the IRAK family, IRAK4 is recruited to receptor complexes via the adaptor protein MyD88 upon TLR/IL-1R activation, triggering downstream signaling cascades that activate NF-κB and MAPK pathways. This leads to the production of pro-inflammatory cytokines and chemokines, essential for host defense against pathogens. IRAK4 deficiency in humans results in recurrent bacterial infections, highlighting its non-redundant role in immunity.
IRAK4 antibodies are widely used as research tools to study its expression, phosphorylation status, and interaction partners in immune cells. They help elucidate IRAK4’s regulatory mechanisms in diseases like autoimmune disorders (e.g., rheumatoid arthritis), chronic inflammation, and cancers, where dysregulated TLR/IL-1R signaling contributes to pathogenesis. IRAK4 inhibitors, including small molecules targeting its kinase domain, are under investigation for therapeutic applications. Antibodies also aid in validating inhibitor specificity and monitoring drug effects in preclinical models. However, IRAK4’s dual role in pro-inflammatory and anti-viral responses necessitates careful targeting to avoid immunosuppressive risks. Ongoing research aims to refine IRAK4 modulation strategies for precision immunotherapy.
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