| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AK7 |
| Uniprot No | Q96M32 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-723aa |
| 氨基酸序列 | MAEEEETAALTEKVIRTQRVFINLLDSYSSGNIGKFLSNCVVGASLEEITEEEEEEDENKSAMLEASSTKVKEGTFQIVGTLSKPDSPRPDFAVETYSAISREDLLMRLLECDVIIYNITESSQQMEEAIWAVSALSEEVSHFEKRKLFILLSTVMTWARSKALDPEDSEVPFTEEDYRRRKSHPNFLDHINAEKMVLKFGKKARKFAAYVVAAGLQYGAEGGMLHTFFKMAWLGEIPALPVFGDGTNVIPTIHVLDLAGVIQNVIDHVPKPHYLVAVDESVHTLEDIVKCISKNTGPGKIQKIPRENAYLTKDLTQDCLDHLLVNLRMEALFVKENFNIRWAAQTGFVENINTILKEYKQSRGLMPIKICILGPPAVGKSSIAKELANYYKLHHIQLKDVISEAIAKLEAIVAPNDVGEGEEEVEEEEEEENVEDAQELLDGIKESMEQNAGQLDDQYIIRFMKEKLKSMPCRNQGYILDGFPKTYDQAKDLFNQEDEEEEDDVRGRMFPFDKLIIPEFVCALDASDEFLKERVINLPESIVAGTHYSQDRFLRALSNYRDINIDDETVFNYFDELEIHPIHIDVGKLEDAQNRLAIKQLIKEIGEPRNYGLTDEEKAEEERKAAEERLAREAAEEAEREHQEAVEMAEKIARWEEWNKRLEEVKREERELLEAQSIPLRNYLMTYVMPTLIQGLNECCNVRPEDPVDFLAEYLFKNNPEAQ |
| 预测分子量 | 83.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AK7重组蛋白的参考文献示例(内容为虚构，仅供参考)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant AK7 in Mitochondrial Metabolism*
**作者**: Zhang, L., et al. (2020)
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了AK7重组蛋白，验证其腺苷酸激酶活性。实验表明AK7参与线粒体ATP/AMP动态平衡调控，缺失AK7导致细胞能量代谢异常。
2. **文献名称**: *Structural Insights into AK7 Catalytic Mechanism by X-ray Crystallography*
**作者**: Tanaka, K., et al. (2018)
**摘要**: 通过X射线晶体学解析AK7重组蛋白的三维结构(分辨率2.1Å)，揭示了其底物结合位点的关键氨基酸残基，为靶向药物设计提供结构基础。
3. **文献名称**: *AK7 Knockout Mice Exhibit Neurodegenerative Phenotypes*
**作者**: Smith, J., et al. (2019)
**摘要**: 构建AK7基因敲除小鼠模型，发现其运动神经元退行性病变加剧，提示AK7可能在神经保护中发挥作用，并与肌萎缩侧索硬化症(ALS)相关通路关联。
4. **文献名称**: *AK7 as a Potential Biomarker in Colorectal Cancer*
**作者**: Chen, X., et al. (2021)
**摘要**: 在结直肠癌组织中检测到AK7表达显著上调，体外实验表明抑制AK7重组蛋白活性可降低癌细胞增殖，提示其作为治疗靶点的潜力。
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注：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
AK7 recombinant protein is derived from adenylate kinase 7 (AK7), a member of the adenylate kinase family that plays a critical role in cellular energy metabolism by catalyzing the reversible transfer of phosphate groups between nucleotides (e.g., ATP, ADP, and AMP). This enzyme is primarily localized in mitochondria and cytoplasm, contributing to nucleotide homeostasis and energy signaling pathways. AK7 is implicated in diverse biological processes, including cell proliferation, differentiation, and apoptosis, with dysregulation linked to metabolic disorders, neurodegenerative diseases, and certain cancers.
The recombinant AK7 protein is produced using genetic engineering techniques, typically expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Its production enables researchers to study AK7’s enzymatic activity, structural characteristics, and interactions with other biomolecules in vitro. Recombinant AK7 serves as a vital tool for elucidating its physiological roles, such as modulating mitochondrial function or influencing nucleotide-dependent signaling cascades.
Recent studies highlight AK7’s potential as a therapeutic target. For instance, altered AK7 expression has been observed in conditions like hepatocellular carcinoma and Parkinson’s disease, suggesting its involvement in disease pathogenesis. Additionally, recombinant AK7 facilitates drug screening by providing a purified protein substrate for testing inhibitors or activators. Despite progress, challenges remain in fully understanding its tissue-specific functions and regulatory mechanisms. Ongoing research aims to unravel AK7’s role in metabolic adaptability and stress responses, which could inform novel treatment strategies for energy-related disorders.
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