| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | STAT5; STAT5B |
| Entrez GeneID | 6777 |
| clone | 5B3 |
| WB Predicted band size | 90kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human STAT5B expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于STAT5B抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*STAT5B mutations in hematopoietic malignancies*
**作者**：Van Etten, R.A. 等
**摘要**：该研究揭示了STAT5B基因突变在白血病和淋巴瘤中的致癌作用，并利用特异性抗体检测突变型STAT5B的异常激活，证明其通过持续激活JAK-STAT信号通路促进肿瘤发生。
2. **文献名称**：*Role of STAT5B in immune cell regulation*
**作者**：Kitamura, T. 等
**摘要**：通过STAT5B特异性抗体阻断实验，研究发现STAT5B在T细胞和自然杀伤细胞(NK细胞)的分化及功能中起关键作用，其缺失导致免疫应答显著减弱。
3. **文献名称**：*STAT5B phosphorylation in breast cancer progression*
**作者**：Hennighausen, L. 等
**摘要**：该文献利用STAT5B抗体进行免疫组化分析，发现STAT5B的磷酸化水平与乳腺癌侵袭性呈正相关，提示其可作为预后标志物及治疗靶点。
4. **文献名称**：*STAT5B-specific antibodies reveal isoform-selective signaling*
**作者**：Pear, W.S. 等
**摘要**：研究通过开发高特异性STAT5B抗体，揭示了STAT5B与STAT5A在信号传导中的功能差异，尤其在细胞增殖与凋亡调控中的独特作用。
(注：上述文献为示例性质，实际引用时需核实具体文献信息。)
The STAT5B antibody is a crucial tool in studying the STAT (Signal Transducer and Activator of Transcription) protein family, specifically targeting the STAT5B isoform. STAT5B, along with STAT5A, is a transcription factor activated by cytokine, hormone, and growth factor signaling via the JAK-STAT pathway. Upon receptor ligation, STAT5B is phosphorylated at tyrosine residues (e.g., Y699), leading to dimerization, nuclear translocation, and regulation of target genes involved in cell proliferation, differentiation, survival, and immune responses. STAT5B shares ~96% amino acid identity with STAT5A but exhibits distinct roles in immune regulation, metabolism, and hematopoiesis, with STAT5B mutations linked to immune dysregulation, growth disorders, and cancer.
STAT5B antibodies are widely used in research to detect protein expression, phosphorylation status, and localization in tissues or cells. They enable techniques like Western blotting, immunohistochemistry, flow cytometry, and chromatin immunoprecipitation (ChIP). Dysregulated STAT5B signaling is implicated in leukemias, lymphomas, and autoimmune diseases, making these antibodies vital for mechanistic studies and therapeutic development. Validation of STAT5B antibodies requires specificity checks using knockout controls or siRNA, as cross-reactivity with STAT5A is common. Commercial antibodies often target conserved regions (e.g., C-terminus) or phospho-specific epitopes to distinguish active isoforms. Research on STAT5B continues to uncover its tissue-specific functions and potential as a biomarker or drug target in precision medicine.
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