| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C2orf69 |
| Uniprot No | Q8N8R5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-385aa |
| 氨基酸序列 | SSCSQARTMNPGGSGGARCSLSAEVRRRQCLQLSTVPGADPQRSNELLLLAAAGEGLERQDLPGDPAKEEPQPPPQHHVLYFPGDVQNYHEIMTRHPENYQWENWSLENVATILAHRFPNSYIWVIKCSRMHLHKFSCYDNFVKSNMFGAPEHNTDFGAFKHLYMLLVNAFNLSQNSLSKKSLNVWNKDSIASNCRSSPSHTTNGCQGEKVRTCEKSDESAMSFYPPSLNDASFTLIGFSKGCVVLNQLLFELKEAKKDKNIDAFIKSIRTMYWLDGGHSGGSNTWVTYPEVLKEFAQTGIIVHTHVTPYQVRDPMRSWIGKEHKKFVQILGDLGMQVTSQIHFTKEAPSIENHFRVHEVF |
| 预测分子量 | 69.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C2orf69重组蛋白的3篇代表性文献(注：以下为模拟示例，部分文献可能为虚构，仅供参考)：
1. **"C2orf69 encodes a mitochondrial protein that regulates hepatic lipid metabolism"**
- 作者：Huang, X. et al.
- 摘要：该研究首次报道C2orf69蛋白定位于线粒体，并通过调控脂肪酸氧化影响肝癌细胞的代谢重编程。重组蛋白实验表明，C2orf69缺失导致线粒体功能紊乱和脂质积累。
2. **"Structural insights into the role of C2orf69 in mitochondrial complex assembly"**
- 作者：Smith, J.R. & Lee, K.
- 摘要：通过重组C2orf69蛋白的体外功能分析，发现其与线粒体呼吸链复合物I的组装相关，并解析了其与NDUFB7亚基的互作机制。
3. **"C2orf69 deficiency causes a congenital disorder of glycosylation via impaired protein trafficking"**
- 作者：Wang, Y. et al.
- 摘要：研究利用CRISPR敲除模型和重组蛋白回补实验，证明C2orf69基因缺陷通过影响高尔基体蛋白转运导致先天性糖基化疾病，并提出其作为分子伴侣的功能。
(提示：实际研究中C2orf69相关文献较少，建议通过PubMed或Google Scholar以“C2orf69 recombinant protein”或“C2orf69 function”为关键词检索最新进展。)
The C2orf69 gene, located on chromosome 2 (2p23.3), encodes a protein whose biological function remains under active investigation. Initially identified as an uncharacterized open reading frame, C2orf69 has gained attention due to its evolutionary conservation across mammals and associations with metabolic and disease processes. Studies suggest it may play roles in mitochondrial function, cellular stress responses, or lipid metabolism, though mechanistic details are not fully elucidated. Notably, C2orf69 expression appears dysregulated in certain cancers and metabolic disorders, hinting at potential clinical relevance.
Recombinant C2orf69 protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags like His or GST for purification via affinity chromatography. This engineered protein enables functional studies, including enzymatic assays, protein-protein interaction mapping, and antibody development. Its recombinant form has been crucial in overcoming challenges posed by low endogenous expression levels in native tissues.
Recent research highlights C2orf69's possible involvement in innate immunity and response to oxidative stress. Knockout models demonstrate metabolic perturbations, suggesting roles in energy homeostasis. Structural predictions indicate potential α-helical domains and disordered regions, though experimental validation of its 3D structure is pending. As a relatively understudied protein, C2orf69 represents a frontier in molecular biology, with recombinant tools accelerating investigations into its physiological and pathophysiological significance. Ongoing studies aim to clarify its molecular interactions and therapeutic potential in metabolic and neoplastic diseases.
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