| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Gna12 |
| Uniprot No | P27600 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-379aa |
| 氨基酸序列 | MSGVVRTLSRCLLPAEAGARERRAGAARDAEREARRRSRDIDALLARERRAVRRLVKILLLGAGESGKSTFLKQMRIIHGREFDQKALLEFRDTIFDNILKGSRVLVDARDKLGIPWQHSENEKHGMFLMAFENKAGLPVEPATFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYFPSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSSEYDQVLMEDRRTNRLVESMNIFETIVNNKLFFNVSIILFLNKMDLLVEKVKSVSIKKHFPDFKGDPHRLEDVQRYLVQCFDRKRRNRSKPLFHHFTTAIDTENIRFVFHAVKDTILQENLKDIMLQ |
| 预测分子量 | 51.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Gna12重组蛋白的3篇代表性文献的简要信息(注：文献信息为模拟示例，具体引用需以实际论文为准)：
1. **文献名称**：*"Gα12 regulates metastatic progression via interaction with integrin β1 in breast cancer"*
**作者**：Smith A, et al.
**摘要**：研究发现Gna12重组蛋白通过与整合素β1相互作用激活下游RhoA信号通路，促进乳腺癌细胞的迁移和侵袭，提示其可能作为肿瘤转移的潜在治疗靶点。
2. **文献名称**：*"Recombinant Gα12 protein induces cytoskeletal remodeling through Rho GTPase activation"*
**作者**：Chen L, et al.
**摘要**：该研究利用重组Gna12蛋白体外实验证实，其可通过激活Rho家族GTP酶(如RhoA和Cdc42)引发细胞骨架重组，揭示了Gna12在细胞形态调控中的直接作用机制。
3. **文献名称**：*"Gna12-mediated signaling promotes liver fibrosis in a mouse model"*
**作者**：Kimura T, et al.
**摘要**：通过构建肝纤维化小鼠模型，作者发现重组Gna12蛋白表达上调会加剧肝星状细胞活化及胶原沉积，表明Gna12可能参与纤维化疾病的发展。
(注：如需真实文献，建议通过PubMed或Google Scholar检索关键词“GNA12 recombinant protein”或“Gα12 signaling”。)
**Background of GNA12 Recombinant Protein**
GNA12 (Guanine Nucleotide-Binding Protein Subunit Alpha-12) is a member of the Gα12/13 subfamily of heterotrimeric G-protein α subunits, which transduce signals from G protein-coupled receptors (GPCRs) to downstream effectors. It plays a critical role in regulating cellular processes such as cytoskeletal remodeling, cell migration, proliferation, and apoptosis. GNA12 is activated by receptor-mediated exchange of GDP for GTP, triggering interactions with effectors like RhoGEFs to modulate Rho GTPase pathways. Dysregulation of GNA12 signaling is implicated in pathologies including cancer metastasis, cardiovascular disorders, and neurological diseases.
Recombinant GNA12 protein is engineered for in vitro studies to dissect its molecular functions, interactions, and signaling mechanisms. Produced via heterologous expression systems (e.g., *E. coli*, mammalian cells*), it retains functional domains required for GTP binding/hydrolysis and effector engagement. Purification tags (e.g., His-tag) facilitate isolation, while quality controls (SDS-PAGE, activity assays) ensure structural integrity and bioactivity.
Researchers utilize recombinant GNA12 to study its role in disease models, screen therapeutic agents targeting aberrant Gα12 signaling, or explore structural features via crystallography. Its availability accelerates mechanistic insights into GPCR-mediated pathways and supports drug discovery for conditions linked to GNA12 dysregulation.
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