| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | SWD3; BIG-3; WDR5 |
| Entrez GeneID | 11091 |
| clone | 7B11 |
| WB Predicted band size | 36.6kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human WDR5 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于WDR5抗体的3篇参考文献示例(内容基于虚构的文献模拟，仅供参考格式)：
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1. **文献名称**: *WDR5 mediates histone H3 lysine 4 methylation and oncogenesis in acute myeloid leukemia*
**作者**: Smith J, Brown K, et al.
**摘要**: 本研究利用WDR5特异性抗体(货号：AB123)通过染色质免疫沉淀(ChIP)和Western blot技术，揭示了WDR5在急性髓系白血病(AML)中通过调控组蛋白H3K4甲基化促进癌基因表达的机制。研究证实WDR5与MLL复合物的相互作用对白血病细胞增殖至关重要。
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2. **文献名称**: *Targeting WDR5-MYC interaction suppresses tumor growth in colorectal cancer*
**作者**: Li X, Chen Z, et al.
**摘要**: 通过免疫共沉淀(Co-IP)和免疫荧光实验(使用WDR5抗体，品牌：Cell Signaling #1234)，本研究证明WDR5与MYC蛋白直接结合，调控结直肠癌细胞中促癌基因的转录。抑制该相互作用可显著抑制肿瘤生长，为靶向治疗提供了新策略。
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3. **文献名称**: *Structural basis of WDR5 interactions with RNA polymerase II*
**作者**: Garcia R, Patel S, et al.
**摘要**: 结合X射线晶体学和Western blot分析(采用抗WDR5抗体，Sigma-Aldrich WDR5-1A)，本研究解析了WDR5与RNA聚合酶II结合的分子机制，揭示了其在转录延伸阶段的关键作用，为表观遗传调控提供了结构生物学证据。
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**注**：以上文献为示例，实际引用时需根据真实发表的论文调整。建议通过PubMed或Google Scholar搜索关键词“WDR5 antibody”、“WDR5 ChIP”或“WDR5 protein interaction”获取真实文献。
WDR5 (WD Repeat Domain 5) is a conserved nuclear protein belonging to the WD40-repeat protein family, known for its role in epigenetic regulation. It serves as a core subunit of multiple histone-modifying complexes, including the MLL/COMPASS and SET1 methyltransferase complexes, which catalyze histone H3 lysine 4 methylation (H3K4me), a hallmark of transcriptionally active chromatin. WDR5 facilitates complex assembly by bridging interactions between catalytic subunits (e.g., MLL1) and other regulatory proteins, thereby influencing gene expression, cell differentiation, and developmental processes. Dysregulation of WDR5 has been implicated in cancers, particularly leukemias, due to its role in maintaining oncogenic gene programs.
WDR5 antibodies are essential tools for studying its function, localization, and interactions. They are widely used in techniques like Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to detect WDR5 expression levels, binding partners, and chromatin occupancy. High-quality WDR5 antibodies exhibit specificity for distinct epitopes, enabling researchers to differentiate between isoforms or post-translationally modified forms. Validation methods often include knockout cell lines or competitive peptide blocking to confirm target specificity. Challenges in antibody development arise from WDR5’s structural conservation across species and its participation in diverse multiprotein complexes. Reliable WDR5 antibodies are critical for advancing research in epigenetics, cancer biology, and targeted therapies aiming to disrupt WDR5-dependent oncogenic pathways.
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