Mouse Monoclonal TCF3 Antibody

The TCF3 antibody targets the Transcription Factor 3 (TCF3), also known as E2A, encoded by the *TCF3* gene in humans. TCF3 belongs to the E-protein family of basic helix-loop-helix (bHLH) transcription factors, which play critical roles in cell differentiation, embryonic development, and immune system regulation. TCF3 is essential for B-cell and T-cell maturation, governing the expression of genes involved in immunoglobulin production and lymphocyte development. It forms homodimers or heterodimers with other bHLH proteins to regulate transcription by binding E-box motifs in DNA. Dysregulation of TCF3 is linked to malignancies, particularly acute lymphoblastic leukemia (ALL), where chromosomal translocations (e.g., t(1;19)) generate oncogenic fusion proteins like E2A-PBX1. TCF3 antibodies are widely used in research to study its expression, localization, and interactions in normal and pathological contexts. These antibodies enable detection via techniques such as Western blotting, immunohistochemistry, and flow cytometry. Specific clones may distinguish between TCF3 isoforms (E12/E47) or post-translational modifications. Their applications span immunology, oncology, and developmental biology, aiding in understanding TCF3's dual roles as a tumor suppressor and oncogenic driver, depending on cellular context. Validation of TCF3 antibodies ensures specificity for accurate experimental outcomes.