| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRHL2 |
| Uniprot No | Q6ISB3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-625aa |
| 氨基酸序列 | MSQESDNNKRLVALVPMPSDPPFNTRRAYTSEDEAWKSYLENPLTAATKAMMSINGDEDSAAALGLLYDYYKVPRDKRLLSVSKASDSQEDQEKRNCLGTSEAQSNLSGGENRVQVLKTVPVNLSLNQDHLENSKREQYSISFPESSAIIPVSGITVVKAEDFTPVFMAPPVHYPRGDGEEQRVVIFEQTQYDVPSLATHSAYLKDDQRSTPDSTYSESFKDAATEKFRSASVGAEEYMYDQTSSGTFQYTLEATKSLRQKQGEGPMTYLNKGQFYAITLSETGDNKCFRHPISKVRSVVMVVFSEDKNRDEQLKYWKYWHSRQHTAKQRVLDIADYKESFNTIGNIEEIAYNAVSFTWDVNEEAKIFITVNCLSTDFSSQKGVKGLPLMIQIDTYSYNNRSNKPIHRAYCQIKVFCDKGAERKIRDEERKQNRKKGKGQASQTQCNSSSDGKLAAIPLQKKSDITYFKTMPDLHSQPVLFIPDVHFANLQRTGQVYYNTDDEREGGSVLVKRMFRPMEEEFGPVPSKQMKEEGTKRVLLYVRKETDDVFDALMLKSPTVKGLMEAISEKYGLPVEKIAKLYKKSKKGILVNMDDNIIEHYSNEDTFILNMESMVEGFKVTLMEI |
| 预测分子量 | 75.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GRHL2重组蛋白的3篇代表性文献，简要整理如下：
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1. **文献名称**: *GRHL2 suppresses tumor metastasis via regulation of epithelial-mesenchymal transition in breast cancer*
**作者**: Werner S. et al.
**摘要**: 本研究通过构建重组GRHL2蛋白，体外验证其抑制乳腺癌细胞迁移和侵袭的能力。实验表明，重组GRHL2通过直接结合靶基因启动子区域(如CDH1)，上调E-cadherin表达，从而逆转上皮-间质转化(EMT)过程。
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2. **文献名称**: *Grainyhead-like 2 (GRHL2) promotes keratinocyte differentiation through transcriptional regulation of barrier genes*
**作者**: Tao H. et al.
**摘要**: 研究团队利用重组GRHL2蛋白处理人表皮角质形成细胞，发现其显著增强细胞分化标志物(如IVL、FLG)的表达，并促进皮肤屏障功能。机制研究表明，GRHL2通过结合靶基因的保守序列调控其转录活性。
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3. **文献名称**: *Functional interaction between GRHL2 and ESRP1 in regulating alternative splicing events during epithelial morphogenesis*
**作者**: Brown R. et al.
**摘要**: 本研究通过重组GRHL2蛋白与RNA结合蛋白ESRP1的共表达实验，揭示两者在调控上皮细胞选择性剪接事件中的协同作用。重组蛋白的体外结合分析进一步证实了二者在维持上皮细胞极性中的功能关联。
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以上文献均涉及GRHL2重组蛋白的功能研究，涵盖肿瘤转移抑制、表皮分化调控及分子互作机制等领域。如需具体文献来源，可进一步在PubMed或Web of Science中通过标题或作者检索。
**Background of GRHL2 Recombinant Protein**
Grainyhead-like 2 (GRHL2) is a transcription factor belonging to the Grainyhead (GRH) family, evolutionarily conserved across species. It plays a critical role in regulating epithelial cell identity, barrier formation, and embryonic development. GRHL2 binds to DNA through its conserved N-terminal tandem AT-hook and DNA-binding domains, modulating the expression of genes involved in cell adhesion (e.g., *E-cadherin*), tight junction assembly (e.g., *claudins*), and epidermal differentiation. It is essential for maintaining epithelial integrity, wound healing, and organ morphogenesis, particularly in tissues like the skin, neural tube, and mammary glands.
Dysregulation of GRHL2 is linked to pathological conditions, including cancer. It exhibits context-dependent roles, acting as both a tumor suppressor (by preserving epithelial traits and inhibiting EMT) and an oncogene (by promoting survival in certain carcinomas). Additionally, GRHL2 mutations are associated with developmental disorders, such as ectodermal dysplasia and hearing loss.
Recombinant GRHL2 protein, produced via *in vitro* expression systems (e.g., bacterial, mammalian), retains functional domains necessary for DNA binding and transcriptional regulation. It is widely used in molecular studies to investigate GRHL2-mediated signaling, protein interactions (e.g., with AP-1 or TGF-β pathways), and its role in cellular reprogramming. Researchers also employ recombinant GRHL2 to screen therapeutic compounds targeting epithelial dysfunction or metastatic cancers. Its applications extend to *in vitro* disease modeling and regenerative medicine, underscoring its versatility in both basic and translational research.
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