| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MEK2; MKK2; MAPKK2 |
| Entrez GeneID | 5605 |
| clone | 7F5 |
| WB Predicted band size | 44kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human MAP2K2 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇模拟的关于MAP2K2抗体的参考文献示例(注：文献信息为虚构，仅供示例参考)：
1. **《MAP2K2抗体在黑色素瘤细胞信号通路研究中的应用》**
- 作者：Smith A, et al.
- 摘要：本研究使用特异性MAP2K2抗体，通过Western blot和免疫组化技术验证了MAP2K2蛋白在黑色素瘤细胞中的高表达，并发现其与ERK通路的持续激活相关，为靶向治疗提供依据。
2. **《Development of a High-Affinity MEK2 (MAP2K2) Monoclonal Antibody for Functional Studies》**
- 作者：Lee JH, et al.
- 摘要：报道了一种高特异性MAP2K2单克隆抗体的开发，通过免疫沉淀和激酶活性实验证实该抗体可有效抑制MEK2-ERK相互作用，为信号转导机制研究提供工具。
3. **《MAP2K2 Expression Profiling in Lung Adenocarcinoma Using a Novel Validated Antibody》**
- 作者：Wang Y, et al.
- 摘要：利用新验证的MAP2K2多克隆抗体分析肺癌组织样本，发现MAP2K2表达与EGFR突变状态显著相关，提示其作为预后标志物的潜在价值。
4. **《MEK Inhibitor Resistance Mechanisms Revealed by MAP2K2 Antibody-Based Screening》**
- 作者：Garcia R, et al.
- 摘要：通过MAP2K2抗体的免疫荧光染色和流式细胞术，揭示了结直肠癌细胞对MEK抑制剂司美替尼的耐药性与MAP2K2蛋白异构体表达上调有关。
**备注**：以上文献为模拟内容，实际研究中建议通过PubMed或Google Scholar检索真实文献(关键词：MAP2K2 antibody, MEK2. ERK pathway)。
The MAP2K2 antibody targets mitogen-activated protein kinase kinase 2 (MAP2K2), also known as MEK2. a dual-specificity kinase within the RAS/MAPK signaling pathway. This pathway regulates critical cellular processes, including proliferation, differentiation, and apoptosis. MAP2K2 functions downstream of RAS and RAF kinases, activating MAPK1/ERK2 and MAPK3/ERK1 through phosphorylation. Dysregulation of MAP2K2 is linked to cancers (e.g., melanoma, colorectal), developmental disorders (e.g., Noonan syndrome), and autoimmune conditions.
MAP2K2 antibodies are essential tools in research and diagnostics. They detect protein expression, localization, and activation status in tissues or cell lines, often via Western blot, immunohistochemistry, or immunofluorescence. These antibodies help study mutations (e.g., those conferring resistance to MEK inhibitors) or pathway hyperactivity in diseases. Additionally, they aid in evaluating therapeutic responses to targeted inhibitors in oncology.
Structurally, MAP2K2 contains a catalytic domain and a nuclear localization signal. Antibodies often recognize specific epitopes, phosphorylated residues (e.g., T394), or conserved regions to ensure specificity. As the RAS/MAPK pathway is a major drug target, MAP2K2 antibodies also support biomarker discovery and drug development. Their applications span basic research, clinical diagnostics, and translational studies, underscoring their importance in understanding disease mechanisms and advancing precision therapies.
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