| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | bcd |
| Uniprot No | P09081 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-494aa |
| 氨基酸序列 | MAQPPPDQNFYHHPLPHTHTHPHPHSHPHPHSHPHPHHQHPQLQLPPQFRNPFDLLFDERTGAINYNYIRPYLPNQMPKPDVFPSEELPDSLVMRRPRRTRTTFTSSQIAELEQHFLQGRYLTAPRLADLSAKLALGTAQVKIWFKNRRRRHKIQSDQHKDQSYEGMPLSPGMKQSDGDPPSLQTLSLGGGATPNALTPSPTPSTPTAHMTEHYSESFNAYYNYNGGHNHAQANRHMHMQYPSGGGPGPGSTNVNGGQFFQQQQVHNHQQQLHHQGNHVPHQMQQQQQQAQQQQYHHFDFQQKQASACRVLVKDEPEADYNFNSSYYMRSGMSGATASASAVARGAASPGSEVYEPLTPKNDESPSLCGIGIGGPCAIAVGETEAADDMDDGTSKKTTLQILEPLKGLDKSCDDGSSDDMSTGIRALAGTGNRGAAFAKFGKPSPPQGPQPPLGMGGVAMGESNQYQCTMDTIMQAYNPHRNAAGNSQFAYCFN |
| 预测分子量 | 54,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCD重组蛋白的3篇参考文献示例，内容基于领域内常见研究方向模拟生成：
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**1. 文献名称**：*Expression and Purification of Recombinant Bicoid (BCD) Protein for DNA Binding Studies*
**作者**：Schneider, D., et al.
**摘要**：本研究报道了通过原核表达系统高效表达果蝇Bicoid(BCD)重组蛋白的优化方法，利用His标签亲和层析技术纯化获得高纯度蛋白。功能实验证实重组BCD蛋白能够特异性结合靶DNA序列，并激活下游报告基因表达，为研究其转录调控机制提供材料基础。
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**2. 文献名称**：*Structural Analysis of BCD Homeodomain Using Recombinant Protein Crystallography*
**作者**：Tanaka, M., & Patel, R.
**摘要**：通过重组表达BCD蛋白的同源结构域(homeodomain)，解析了其高分辨率晶体结构，揭示了关键氨基酸残基在DNA结合中的构象变化，阐明了BCD蛋白靶向特定基因启动子的分子机制。
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**3. 文献名称**：*Functional Rescue of BCD Mutant Phenotypes by Recombinant Protein Microinjection*
**作者**：Weber, J., et al.
**摘要**：在果蝇胚胎中显微注射重组BCD蛋白，成功恢复了bcd基因突变体的胚胎前后轴发育缺陷，证明重组蛋白在体内的生物学活性，为研究形态发生梯度形成提供了直接证据。
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**注**：以上文献为模拟内容，实际研究中建议通过学术数据库(如PubMed、Web of Science)以“Bicoid recombinant protein”或“BCD protein expression”等关键词检索最新文献。
**Background of BCD Recombinant Proteins**
BCD recombinant proteins are engineered molecules typically designed by fusing specific functional domains, often including modular domains like Bcl-2 homology (BH), coiled-coil, or other interaction motifs, to achieve targeted biological functions. The term "BCD" may refer to a combination of domains or a specific tripartite motif (e.g., Bcl-2. caspase, death effector domain) involved in critical cellular processes such as apoptosis, immune signaling, or cell cycle regulation. These proteins are produced using recombinant DNA technology, where genes encoding desired domains are cloned, expressed, and purified in host systems like *E. coli* or mammalian cells.
The development of BCD recombinant proteins is driven by the need to study complex protein interactions, signaling pathways, or therapeutic targets. For example, in cancer research, BCD proteins mimicking pro-apoptotic factors help elucidate mechanisms of cell death or resistance. Their modular design allows customization for binding specificity, stability, or delivery efficiency, making them valuable tools in drug discovery, structural biology, and functional assays.
Applications extend to therapeutics, where engineered BCD proteins act as biologics to modulate disease pathways, such as inhibiting oncogenic proteins or enhancing immune responses. Advances in protein engineering, like CRISPR-based editing or computational modeling, further refine their design. Challenges include ensuring proper folding, minimizing immunogenicity, and achieving in vivo efficacy. Overall, BCD recombinant proteins represent a versatile platform bridging basic research and translational medicine, offering insights into cellular mechanisms and potential treatments for diseases ranging from cancer to autoimmune disorders.
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