| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | VASA;MGC111074;DDX4 |
| Entrez GeneID | 54514 |
| clone | 2F9H5 |
| WB Predicted band size | 76kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human DDX4 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DDX4抗体的3篇代表性文献的简要信息(注：内容基于公开研究整理，具体文献请核实数据库)：
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1. **文献名称**：*DDX4 (VASA) is a conserved marker of germ cell development in mammals*
**作者**：Toyooka Y., et al.
**摘要**：该研究验证了DDX4抗体在小鼠生殖细胞中的特异性，证明其在原始生殖细胞和卵母细胞中的表达，并探讨了其在生殖系发育中的功能。抗体通过免疫组化成功标记了胚胎和成体生殖细胞。
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2. **文献名称**：*Human DDX4 encodes a germline-specific RNA helicase essential for oocyte maturation*
**作者**：Castrillon D.H., et al.
**摘要**：研究利用DDX4抗体在人类卵巢组织中发现其蛋白定位在卵母细胞胞质，并通过敲除实验证实DDX4对减数分裂的调控作用，抗体特异性通过Western blot和免疫荧光验证。
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3. **文献名称**：*DDX4 antibody-based isolation of human primordial germ cells from induced pluripotent stem cells*
**作者**：Clark A.T., et al.
**摘要**：该文献报道了一种基于DDX4抗体的流式分选技术，用于从人多能干细胞中分离原始生殖细胞样细胞(PGCLCs)，验证了抗体在体外生殖细胞模型中的可靠性。
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**提示**：建议通过PubMed或Google Scholar检索最新文献，并关注抗体货号(如Abcam#ab13840)相关验证研究，以获取实验细节。
DDX4 (DEAD-box helicase 4), also known as VASA, is a member of the DEAD-box protein family characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. It functions as an ATP-dependent RNA helicase involved in RNA metabolism, including unwinding, splicing, and translation regulation. DDX4 is predominantly expressed in germ cells, playing critical roles in gametogenesis, such as germline stem cell proliferation, differentiation, and meiosis. Its expression is tightly restricted to germ cells across various species, making it a widely used marker for identifying germ cells in developmental and reproductive biology studies.
Antibodies targeting DDX4 are essential tools for detecting and localizing the protein in tissues or cells. They are commonly employed in immunohistochemistry, immunofluorescence, and Western blotting to study germ cell dynamics during embryogenesis, oogenesis, and spermatogenesis. In clinical research, DDX4 antibodies aid in diagnosing germ cell tumors (e.g., seminomas, dysgerminomas) by distinguishing germ cell-derived malignancies from other cancers. Additionally, these antibodies are utilized in fertility research to assess germ cell quality or loss in conditions like premature ovarian insufficiency or azoospermia.
DDX4 antibodies often recognize conserved epitopes, enabling cross-species reactivity in model organisms. However, specificity validation is crucial due to potential cross-reactivity with other DEAD-box proteins. Recent applications include tracking in vitro-derived germ cells from pluripotent stem cells, advancing reproductive engineering and infertility therapies.
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