Mouse Monoclonal eNOS Antibody

Endothelial nitric oxide synthase (eNOS) antibodies are essential tools for studying the expression, regulation, and function of eNOS, a key enzyme encoded by the *NOS3* gene. eNOS, primarily expressed in vascular endothelial cells, catalyzes the production of nitric oxide (NO), a critical signaling molecule involved in vasodilation, blood pressure regulation, and vascular homeostasis. Dysregulation of eNOS activity or expression is linked to cardiovascular diseases, including hypertension, atherosclerosis, and diabetes-related vascular complications. eNOS antibodies are widely used in biomedical research to detect eNOS protein levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help identify eNOS localization in tissues, assess post-translational modifications (e.g., phosphorylation at Ser1177 or Thr495), and study interactions with regulatory proteins. Polyclonal and monoclonal eNOS antibodies are available, often raised against specific epitopes in human or murine eNOS. Researchers also utilize these antibodies to explore eNOS dysfunction in disease models, evaluate therapeutic interventions, and validate genetic knockout models. However, specificity validation (e.g., using knockout controls) is critical due to potential cross-reactivity with other NOS isoforms (nNOS, iNOS). Overall, eNOS antibodies remain indispensable for unraveling the enzyme's role in vascular biology and pathophysiology.