| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | alpha-fetoprotein; FETA; HPAFP |
| Entrez GeneID | 174 |
| clone | 6E6 |
| WB Predicted band size | 69kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human AFP expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于AFP(甲胎蛋白)抗体的3篇示例文献(注：以下为模拟文献，建议通过PubMed或Google Scholar获取真实研究)：
1. **文献名称**: *Monoclonal Antibodies Against Alpha-Fetoprotein: Production and Clinical Applications*
**作者**: Smith J, et al.
**摘要**: 研究通过杂交瘤技术制备了高特异性的AFP单克隆抗体，并验证其在肝癌血清学诊断中的敏感性(92%)和特异性(89%)，提出其在肿瘤早期筛查中的潜力。
2. **文献名称**: *AFP-Specific Antibody Engineering for Targeted Cancer Therapy*
**作者**: Chen L, Wang Y.
**摘要**: 通过基因工程技术改造AFP抗体Fc段，开发出具有增强ADCC(抗体依赖细胞毒性)效应的新型抗体，动物实验显示其能显著抑制AFP阳性肝癌移植瘤的生长。
3. **文献名称**: *Autoantibodies to AFP in Chronic Liver Disease: Diagnostic Implications*
**作者**: Gupta R, et al.
**摘要**: 发现慢性肝炎患者血清中存在抗AFP自身抗体(阳性率18%)，可能与肝细胞损伤后AFP异常释放相关，提示其在区分肝癌与非癌性肝病中的辅助诊断价值。
建议通过以下方式获取真实文献：
1. 在PubMed搜索 "AFP antibody diagnosis"
2. 查找《Cancer Research》《Journal of Immunological Methods》等期刊
3. 关注近年关于AFP抗体药物偶联物(ADC)的研究进展
Alpha-fetoprotein (AFP) is a glycoprotein primarily produced during fetal development by the liver and yolk sac. It functions in maintaining osmotic pressure, transporting ligands, and modulating immune responses in the fetus. Postnatally, AFP levels decline sharply, becoming nearly undetectable in healthy adults. Elevated AFP in adults is often associated with pathological conditions, particularly hepatocellular carcinoma (HCC) and germ cell tumors. It also rises during pregnancy, with abnormal levels indicating potential fetal abnormalities like neural tube defects or Down syndrome.
AFP antibodies are immunoglobulins designed to specifically bind AFP, enabling its detection and quantification in clinical and research settings. These antibodies are critical tools in immunoassays such as ELISA, immunohistochemistry, and Western blotting, aiding in the diagnosis and monitoring of AFP-related pathologies. Monoclonal antibodies offer high specificity, targeting a single epitope of AFP, while polyclonal antibodies detect multiple epitopes, enhancing sensitivity.
In clinical practice, AFP antibodies are pivotal for early HCC detection, especially in high-risk populations like chronic hepatitis B/C patients. They also help assess treatment response and recurrence. However, AFP's limited specificity (elevated in non-malignant liver conditions) necessitates complementary biomarkers for accurate diagnosis. Research continues to refine AFP antibody applications, including targeted therapies and imaging probes, to improve oncological management.
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