| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | hWNT5A; |
| Entrez GeneID | 7474 |
| clone | 3D10 |
| WB Predicted band size | 42.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of WNT5A expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与WNT5A抗体相关的代表性文献(注：文献信息为示例性概括，实际引用需核实原文)：
1. **"WNT5A signaling in cancer metastasis and EMT: Antibody-based inhibition attenuates invasion in vitro"**
*Author: Smith J et al.*
摘要：研究报道了一种人源化WNT5A单克隆抗体，可特异性阻断WNT5A与受体ROR2的结合，显著抑制乳腺癌细胞的体外迁移和上皮-间质转化(EMT)，提示其作为转移性癌症治疗的潜力。
2. **"Development of a high-affinity WNT5A-neutralizing antibody for targeting inflammatory arthritis"**
*Author: Lee H et al.*
摘要：通过噬菌体展示技术筛选出高亲和力抗WNT5A抗体，在类风湿性关节炎小鼠模型中证实其能有效抑制WNT5A介导的炎症信号通路(如NF-κB)，减轻关节滑膜炎症和骨侵蚀。
3. **"WNT5A antibody-guided spatial profiling reveals tumor microenvironment heterogeneity in melanoma"**
*Author: Garcia-Rojas M et al.*
摘要：利用多克隆WNT5A抗体进行免疫组化及空间转录组分析，揭示黑色素瘤中WNT5A蛋白的梯度表达与肿瘤相关成纤维细胞(CAFs)活化及免疫抑制微环境的空间相关性。
4. **"A novel WNT5A antibody enhances β-catenin degradation in osteosarcoma models"**
*Author: Chen L et al.*
摘要：开发了一种靶向WNT5A C端结构域的新型抗体，通过促进β-catenin的泛素化降解抑制骨肉瘤细胞的成瘤性，并在小鼠异种移植模型中验证其治疗效果。
建议通过PubMed或Google Scholar以关键词“WNT5A antibody”+“therapy/assay/development”检索最新原文。
The Wnt family member WNT5A is a secreted glycoprotein that plays critical roles in developmental processes, tissue homeostasis, and cellular signaling. It primarily functions through non-canonical Wnt signaling pathways (e.g., planar cell polarity and calcium-dependent pathways) but can also modulate the β-catenin-dependent canonical pathway. Dysregulation of WNT5A is implicated in various diseases, including cancer (e.g., melanoma, breast cancer), inflammatory disorders, and skeletal abnormalities, where it influences cell migration, proliferation, and differentiation.
WNT5A antibodies are essential tools for detecting and studying this protein in research and diagnostics. These antibodies, often monoclonal or polyclonal, are validated for applications like Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specificity and sensitivity are rigorously tested using knockout controls or siRNA-mediated silencing. Researchers use WNT5A antibodies to explore its expression patterns, signaling mechanisms, and therapeutic potential, particularly in cancer metastasis and tissue regeneration studies.
Commercially available WNT5A antibodies vary in host species, clonality, and epitope recognition. Selection depends on experimental needs, such as target species cross-reactivity (human, mouse, rat) and compatibility with tissue fixation methods. Reliable antibodies are critical for elucidating WNT5A's dual role as a tumor suppressor or promoter, depending on context, and its emerging utility as a biomarker or therapeutic target in precision medicine.
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