| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | K5; DDD; EBS2; KRT5A; KRT5 |
| Entrez GeneID | 3852 |
| clone | 7H8 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of Cytokeratin 5 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Cytokeratin(Pan)抗体的3篇代表性文献的简要概括(示例格式，非真实文献)：
1. **文献名称**：*Pan-Cytokeratin Antibodies in Tumor Diagnosis: A Comparative Study*
**作者**：Smith A, et al.
**摘要**：比较了不同克隆号Cytokeratin(Pan)抗体在多种上皮源性肿瘤(如乳腺癌、肺癌)中的免疫组化染色效果，证实其可用于转移癌的鉴别诊断。
2. **文献名称**：*Utility of Cytokeratin Pan-F Antibody in Identifying Circulating Tumor Cells*
**作者**：Lee JH, et al.
**摘要**：研究利用Cytokeratin(Pan)抗体结合流式细胞术检测外周血循环肿瘤细胞，证明其在癌症预后监测中的潜在价值。
3. **文献名称**：*Characterization of Epithelial Cells in Culture Using Pan-Cytokeratin Markers*
**作者**：Moll R, et al.
**摘要**：系统分析了Cytokeratin(Pan)抗体在体外培养细胞中的应用，强调其在区分上皮细胞与间充质细胞中的特异性。
4. **文献名称**：*Cytokeratin Pan Antibody Cross-Reactivity in Non-Epithelial Tissues*
**作者**：Wang Y, et al.
**摘要**：探讨了Cytokeratin(Pan)抗体在非上皮组织(如平滑肌、神经组织)中的潜在交叉反应，提示需结合其他标记物提高诊断准确性。
(注：以上内容为示例性概括，实际文献需通过PubMed/Google Scholar等平台以关键词"Cytokeratin Pan antibody"+"应用方向"检索。)
Cytokeratin (Pan) antibodies are essential tools in diagnostic pathology and research for identifying epithelial cells and their derived neoplasms. Cytokeratins, part of the intermediate filament protein family, are crucial for maintaining cellular structural integrity and are broadly expressed in epithelial tissues. They are classified into type I (acidic) and type II (basic) subtypes, which form heterodimeric complexes. Over 20 cytokeratin subtypes exist, with specific combinations expressed in different epithelial cell types, reflecting their origin and differentiation status.
Pan-cytokeratin antibodies recognize a conserved epitope shared across multiple cytokeratin subtypes, enabling broad detection of epithelial cells regardless of their specific cytokeratin profile. This pan-specificity makes them particularly valuable in distinguishing epithelial tumors (carcinomas) from non-epithelial malignancies (e.g., lymphomas, sarcomas, melanomas) in immunohistochemistry (IHC). They are widely used in tumor diagnosis, metastasis detection, and identifying circulating tumor cells. Additionally, they aid in studying epithelial-mesenchymal transition (EMT) in cancer research.
However, interpretation requires caution, as some non-epithelial cells (e.g., mesothelial cells) may weakly express cytokeratins, and certain carcinomas (e.g., sarcomatoid carcinomas) may lose cytokeratin expression. Despite this, cytokeratin (Pan) antibodies remain indispensable in both clinical diagnostics and experimental studies of epithelial biology and pathology.
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