| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL |
| Entrez GeneID | 960 |
| clone | 8E2F3 |
| WB Predicted band size | 82kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于CD44抗体的经典文献概览(模拟数据，实际引用请核对原文)：
1. **文献名称**: *CD44: From adhesion molecules to signalling regulators*
**作者**: Ponta, H. et al.
**摘要**: 综述CD44分子作为细胞表面黏附受体的多功能性，重点解析其通过结合透明质酸调控细胞迁移、增殖和分化的信号通路机制，并探讨CD44抗体在阻断肿瘤转移中的应用潜力。
2. **文献名称**: *Prospective identification of tumorigenic breast cancer cells using CD44+/CD24− markers*
**作者**: Al-Hajj, M. et al.
**摘要**: 通过流式细胞术筛选CD44+/CD24−表型的乳腺癌细胞，证实其具有干细胞特性及强致瘤性，CD44抗体在此研究中作为关键标记物用于肿瘤干细胞的分离与功能验证。
3. **文献名称**: *Targeting of CD44 eradicates human acute myeloid leukemic stem cells*
**作者**: Jin, L. et al.
**摘要**: 研究发现抗CD44单克隆抗体可通过阻断Wnt/β-catenin通路选择性清除急性髓系白血病干细胞，动物实验显示其显著抑制肿瘤生长，为CD44靶向治疗提供临床前证据。
(注：以上为学术文献模拟描述，真实文献请通过PubMed/Google Scholar检索确认)
CD44 is a transmembrane glycoprotein widely expressed in various cell types, playing critical roles in cell adhesion, migration, signaling, and interaction with the extracellular matrix. It serves as a receptor for hyaluronic acid (HA) and other ligands, participating in physiological processes such as immune response, wound healing, and stem cell maintenance. CD44 exists as multiple isoforms generated by alternative splicing, with the standard isoform (CD44s) and variant isoforms (CD44v) differing in extracellular domain complexity. These isoforms exhibit tissue-specific expression and functional diversity, particularly in cancer biology.
CD44 is notably implicated in cancer progression and metastasis, where its overexpression is associated with enhanced tumor cell invasiveness, stemness, and resistance to therapy. It is a marker for cancer stem cells (CSCs) in various malignancies, including breast, colon, and pancreatic cancers. In the immune system, CD44 regulates lymphocyte activation, homing, and inflammatory responses.
CD44 antibodies are essential tools for research and diagnostics, targeting specific epitopes to study its expression, isoform distribution, and functional roles. Therapeutic CD44-targeting antibodies are under investigation to block pro-tumorigenic signaling or deliver cytotoxic agents to CD44-expressing cancer cells. Challenges include isoform specificity and understanding context-dependent roles in different diseases. Overall, CD44 antibodies hold promise for both mechanistic insights and clinical applications in oncology and immunology.
×
