Mouse Monoclonal LIN28 Antibody

LIN28 antibodies are essential tools for studying the LIN28 protein family, which includes two RNA-binding proteins, LIN28A and LIN28B. These proteins play critical roles in embryonic development, stem cell pluripotency, and metabolism by regulating mRNA translation and microRNA biogenesis, particularly suppressing let-7 miRNA maturation. LIN28A is highly expressed during embryogenesis and in undifferentiated cells, while LIN28B is often linked to cancer progression and poor prognosis. Antibodies targeting LIN28A/B are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression patterns in developmental biology, oncology, and regenerative medicine research. Due to structural similarities between LIN28A and LIN28B, some antibodies may cross-react, necessitating careful validation using appropriate controls (e.g., knockout cells). Commercially available clones include rabbit or mouse monoclonal antibodies recognizing specific epitopes. Researchers frequently employ these antibodies to investigate LIN28's role in tumorigenesis, particularly in cancers with elevated LIN28 levels such as germ cell tumors, hepatocellular carcinoma, and neuroblastoma. Recent studies also explore LIN28 antibodies as potential diagnostic markers for undifferentiated malignancies. Proper antibody selection and validation remain crucial, as expression levels vary significantly across tissues and disease states.