| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CK18; CYK18; KRT18 |
| Entrez GeneID | 3875 |
| clone | 4D11E4 |
| WB Predicted band size | 48kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Cytokeratin 18抗体的3篇参考文献的简要概括：
1. **文献名称**：*"Detection of cytokeratin 18 cleavage by M30 antibody as a marker of apoptosis in colorectal cancer"*
**作者**：Schutte B, et al.
**摘要**：该研究利用M30抗体特异性识别CK18的凋亡相关切割片段(Asp396)，在结直肠癌组织中验证其作为细胞凋亡标志物的有效性，并探讨其在肿瘤治疗反应评估中的应用。
2. **文献名称**：*"Cytokeratin 18 expression pattern correlates with metastatic behavior in breast carcinoma cell lines"*
**作者**：Leers MP, et al.
**摘要**：通过免疫组化分析不同乳腺癌细胞系中CK18的表达差异，发现CK18抗体(如MAB 3480)的染色模式与肿瘤转移潜力相关，提示其可用于评估肿瘤侵袭性。
3. **文献名称**：*"Caspase-cleaved cytokeratin 18 fragment (M30) as a biomarker of drug-induced liver injury"*
**作者**：Bantel H, et al.
**摘要**：研究利用抗CK18的M30抗体检测血清中凋亡特异性CK18片段，证实其在药物性肝损伤(DILI)早期诊断中的敏感性，优于传统肝酶指标。
*注：以上为示例性文献，实际引用时建议通过PubMed或Google Scholar核对具体文献信息。*
Cytokeratin 18 (CK18) is a type I intermediate filament protein belonging to the keratin family, primarily expressed in single-layered epithelial cells and glandular tissues. As a key structural component, it contributes to cellular integrity, mechanical stability, and stress resistance. CK18 pairs with type II keratins (e.g., CK8) to form heterodimeric complexes, critical for maintaining epithelial cell architecture. Its expression is tissue-specific, making it a valuable biomarker for identifying epithelial-derived cells in pathological and research contexts.
CK18 antibodies are widely utilized in immunohistochemistry (IHC) and immunoassays to detect epithelial differentiation in tumors, aiding in the diagnosis of carcinomas (e.g., adenocarcinomas) and distinguishing them from non-epithelial malignancies. Notably, CK18 fragments released during apoptosis (detected via assays like M30) serve as biomarkers for monitoring chemotherapy response or liver injury. In research, these antibodies help study epithelial-mesenchymal transition (EMT), cancer metastasis, and tissue development.
Commercial CK18 antibodies (e.g., clones DC10. Ks18.04) target specific epitopes, with variations in sensitivity across fixation methods. Mutations in the *KRT18* gene are linked to liver and skin disorders, underscoring its physiological relevance. However, cross-reactivity with other keratins requires careful validation in experimental settings. Overall, CK18 antibodies remain indispensable tools in both diagnostic pathology and molecular research.
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