| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | N-CoR; TRAC1; hN-CoR |
| Entrez GeneID | 9611 |
| clone | 7A7A9 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of NCOR1 (aa1-192) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NCOR1抗体的3篇参考文献示例，包含文献名称、作者和摘要概括：
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1. **文献名称**: *NCoR1 is a conserved physiological modulator of muscle mass and oxidative function*
**作者**: Yamamoto H, et al.
**摘要**: 该研究利用NCoR1抗体在小鼠模型中揭示NCoR1通过抑制PPARβ/δ依赖性转录通路调控骨骼肌代谢和氧化功能。实验表明，肌肉特异性敲除NCoR1可增强运动耐力，抗体用于验证蛋白表达水平和复合物结合分析。
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2. **文献名称**: *Nuclear receptor corepressor 1 regulates inflammatory signaling in experimental autoimmune encephalomyelitis*
**作者**: Jia Y, et al.
**摘要**: 研究通过NCoR1抗体检测免疫细胞中NCoR1的表达，发现其在多发性硬化症模型中调控炎症反应。抗体用于Western blot和免疫沉淀，证明NCoR1通过抑制NF-κB通路减轻神经炎症。
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3. **文献名称**: *The corepressor NCoR1 regulates macrophage polarization and lipid metabolism*
**作者**: Mullican SE, et al.
**摘要**: 该文献使用NCoR1抗体分析巨噬细胞极化中的蛋白定位和互作，发现NCoR1缺失导致M2型极化增强和脂质代谢异常。抗体应用于免疫荧光和ChIP-seq，揭示其通过抑制LXR靶基因调控脂质稳态。
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以上研究均依赖NCoR1抗体进行蛋白检测或功能机制分析，涵盖代谢、免疫和神经疾病领域。如需具体年份或期刊信息，可进一步补充检索条件。
The nuclear receptor corepressor 1 (NCOR1) antibody is a critical tool for studying transcriptional regulation and epigenetic mechanisms. NCOR1 is a corepressor protein that interacts with nuclear receptors, such as thyroid hormone receptors and retinoic acid receptors, to repress gene expression by recruiting histone deacetylases (HDACs) and other chromatin-modifying enzymes. It plays a pivotal role in maintaining chromatin condensation, thereby silencing target genes involved in development, metabolism, and homeostasis. Dysregulation of NCOR1 is linked to cancers, metabolic disorders, and neurological diseases.
Antibodies targeting NCOR1 are widely used in techniques like Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate its expression, localization, and protein interactions. These antibodies help identify NCOR1’s role in transcriptional complexes and its dynamic regulation under physiological or pathological conditions.
Most NCOR1 antibodies are raised against specific epitopes, such as the N-terminal or C-terminal regions, and validated for specificity using knockout cell lines or siRNA-mediated knockdown. Researchers rely on these antibodies to explore NCOR1’s interplay with HDAC3. SMRT (NCOR2), and other coregulators, offering insights into therapeutic strategies targeting epigenetic pathways. Proper controls, including isotype-matched IgG and lysate from NCOR1-deficient models, are essential to ensure experimental accuracy.
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