| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HD3; RPD3; RPD3-2 |
| Entrez GeneID | 8841 |
| clone | 3A7B5 |
| WB Predicted band size | 48.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of HDAC3 (aa224-428) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HDAC3抗体的3篇代表性文献，简要概括其研究内容：
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1. **文献名称**：*HDAC3 mediates the repression of *let-7* miRNA during hepatic stellate cell activation*
**作者**：Mann J, et al.
**摘要**：本研究利用HDAC3特异性抗体进行ChIP-seq分析，发现HDAC3通过表观遗传调控抑制 *let-7* miRNA的表达，从而促进肝星状细胞活化和肝纤维化进程。
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2. **文献名称**：*Selective inhibition of HDAC3 targets metabolic vulnerabilities in pancreatic cancer*
**作者**：Lauberth SM, et al.
**摘要**：通过HDAC3抗体免疫沉淀结合质谱分析，揭示了HDAC3与糖代谢相关蛋白的相互作用，证明其特异性抑制剂可抑制胰腺癌细胞能量代谢并诱导凋亡。
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3. **文献名称**：*HDAC3 deletion in microglia alleviates neurodegeneration via histone acetylation-dependent immune modulation*
**作者**：Chen S, et al.
**摘要**：利用HDAC3敲除小鼠模型及抗体验证，发现小胶质细胞中HDAC3通过调控组蛋白乙酰化水平影响神经炎症反应，其缺失可延缓阿尔茨海默病进展。
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以上研究均通过HDAC3抗体进行蛋白定位、互作分析或表观遗传机制解析，涉及纤维化、肿瘤代谢及神经退行性疾病领域。如需具体DOI或年份可补充说明。
**Background of HDAC3 Antibody**
Histone deacetylase 3 (HDAC3) is a member of the HDAC family, which regulates gene expression by removing acetyl groups from histone proteins, leading to chromatin condensation and transcriptional repression. As a Class I HDAC, it plays critical roles in cell cycle progression, differentiation, apoptosis, and metabolic regulation. HDAC3 is also involved in diverse physiological and pathological processes, including cancer, neurodegenerative diseases, and metabolic disorders, making it a key target for epigenetic research.
HDAC3 antibodies are essential tools for studying its expression, localization, and function in biological systems. They are widely used in techniques like Western blotting, immunoprecipitation, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate HDAC3-protein interactions, DNA-binding activity, and subcellular distribution. Validated HDAC3 antibodies are crucial for ensuring specificity, often verified through knockout cell lines or siRNA-mediated knockdown.
These antibodies also aid in exploring HDAC3's role in disease mechanisms and therapeutic development, particularly in evaluating HDAC inhibitors. Researchers rely on high-quality antibodies with cross-reactivity across species (human, mouse, rat) to support translational studies. Proper validation and application of HDAC3 antibodies remain fundamental to advancing epigenetics and targeted therapy research.
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