| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HD3; RPD3; RPD3-2 |
| Entrez GeneID | 8841 |
| clone | 7G6C5 |
| WB Predicted band size | 48.8kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HDAC3 (aa224-428) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HDAC3抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*HDAC3 is a critical component of the corepressor complex that binds the thyroid hormone receptor*
**作者**：Guenther, M. G., Lane, W. S., Fischle, W., et al.
**摘要**：该研究通过免疫沉淀和质谱分析，揭示了HDAC3与核受体共抑制复合体(N-CoR/SMRT)的相互作用，并证明HDAC3的酶活性对甲状腺激素受体介导的转录抑制至关重要。
2. **文献名称**：*Metabolic gene expression through HDAC3-mediated epigenetic regulation*
**作者**：Sun, Z., Feng, D., Fang, B., et al.
**摘要**：研究利用HDAC3抗体进行ChIP-seq和基因敲除实验，发现HDAC3通过调控肝脏中组蛋白去乙酰化，影响代谢相关基因的昼夜节律性表达，从而调节脂质代谢和能量稳态。
3. **文献名称**：*HDAC3 controls intestinal homeostasis through microbiota-dependent and independent mechanisms*
**作者**：Alenghat, T., Osborne, L. C., Saenz, S. A., et al.
**摘要**：通过肠道特异性敲除HDAC3的小鼠模型和免疫组化分析，揭示了HDAC3在维持肠道上皮屏障功能和调控共生菌群中的双重作用，依赖其组蛋白去乙酰化酶活性。
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以上文献涵盖了HDAC3在转录调控、代谢调控及肠道稳态中的关键功能，实验方法均涉及HDAC3抗体的应用(如免疫沉淀、ChIP-seq或免疫组化)。
**Background of HDAC3 Antibody**
Histone deacetylase 3 (HDAC3) is a member of the class I HDAC family, which plays a critical role in epigenetic regulation by removing acetyl groups from histone lysine residues. This activity promotes chromatin condensation, leading to transcriptional repression. HDAC3 is involved in diverse cellular processes, including cell cycle progression, differentiation, metabolism, and apoptosis, and interacts with corepressor complexes such as NCoR/SMRT to regulate gene expression. Dysregulation of HDAC3 has been linked to cancers, neurodegenerative disorders, and metabolic diseases, making it a key therapeutic target.
HDAC3 antibodies are essential tools for studying its expression, localization, and function in biological systems. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP). Specificity and validation are crucial, as HDAC isoforms share structural similarities. High-quality HDAC3 antibodies help elucidate its role in disease mechanisms, assess its interaction with signaling pathways, and evaluate the efficacy of HDAC inhibitors in preclinical models. Researchers rely on these antibodies to advance understanding of epigenetic regulation and develop targeted therapies.
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