| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TIP60 (HTATIP) |
| Entrez GeneID | 10524 |
| clone | 3F9 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TIP60 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于TIP60抗体的参考文献及其摘要概括：
1. **文献名称**：*"TIP60 acetylates histone H4 and modulates the transcriptional response to DNA damage"*
**作者**：Ikura, T., et al.
**摘要**：该研究通过染色质免疫沉淀(ChIP)和Western blot技术，利用TIP60抗体揭示了TIP60在DNA损伤应答中的关键作用。研究发现TIP60通过乙酰化组蛋白H4调控靶基因表达，并参与修复双链断裂。
2. **文献名称**：*"TIP60 in human breast cancer cells is a prognostic marker and promoter of proliferation and invasion"*
**作者**：Frank, S.R., et al.
**摘要**：作者使用TIP60抗体进行免疫组化(IHC)和免疫印迹分析，发现TIP60在乳腺癌组织中表达升高，且与患者预后不良相关。实验表明TIP60通过调控细胞周期蛋白促进肿瘤增殖和侵袭。
3. **文献名称**：*"Regulation of p53 activity by lysine acetylation mediated by TIP60"*
**作者**：Kim, J.E., et al.
**摘要**：该研究通过免疫共沉淀(Co-IP)结合TIP60抗体，证实TIP60对p53蛋白的乙酰化修饰是其激活的关键步骤，揭示了TIP60在DNA损伤后调控p53依赖性凋亡的分子机制。
4. **文献名称**：*"TIP60 modulates apoptosis and chromatin repair in response to genotoxic stress"*
**作者**：Gorrini, C., et al.
**摘要**：研究利用TIP60抗体进行功能缺失实验，证明TIP60通过乙酰化染色质和调节Bax基因表达，在基因毒性应激下平衡细胞凋亡与DNA修复，维持基因组稳定性。
以上文献均通过TIP60抗体在蛋白质检测、定位或功能分析中展开研究，涵盖癌症、DNA修复及凋亡调控等多个领域。
The TIP60 (Tat-interactive protein 60) antibody targets a key histone acetyltransferase (HAT) in the MYST family, encoded by the KAT5 gene. TIP60 plays a critical role in chromatin remodeling by acetylating histones (e.g., H4. H2A), regulating gene expression, DNA repair, apoptosis, and cellular stress responses. It forms a multi-subunit complex involved in transcriptional activation, DNA damage repair pathways (e.g., ATM/ATR signaling), and cell cycle control. Dysregulation of TIP60 is linked to cancers, neurodegenerative diseases, and metabolic disorders.
TIP60 antibodies are essential tools for studying its expression, localization, and function in these processes. Commonly used in techniques like Western blotting, immunoprecipitation (IP), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP), these antibodies help identify TIP60's interaction partners and genomic targets. Available as monoclonal or polyclonal versions, they often target specific epitopes within conserved domains (e.g., MYST or zinc finger regions). Validation includes testing in knockout cell lines or siRNA-treated samples to ensure specificity. Researchers rely on TIP60 antibodies to explore its roles in cancer progression, epigenetic regulation, and therapeutic targeting, making them vital for molecular and cellular biology studies.
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