| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MT-RNR2 |
| Uniprot No | Q8IVG9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-24aa |
| 氨基酸序列 | MAPRGFSCLLLLTSEIDLPVKRRA |
| 预测分子量 | 31.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MT-RNR2重组蛋白相关的文献摘要概括(注：部分文献为虚构示例，实际研究中请核实)：
1. **"Recombinant Humanin protects against mitochondrial dysfunction in Alzheimer's disease models"**
- 作者：Hashimoto, Y. et al.
- 摘要：研究利用重组MT-RNR2编码的Humanin肽，发现其通过抑制线粒体凋亡通路，减轻β-淀粉样蛋白诱导的神经元损伤，为神经退行性疾病治疗提供新策略。
2. **"Expression and purification of MT-RNR2-derived peptide in E. coli for therapeutic applications"**
- 作者：Smith, J. et al.
- 摘要：开发了一种高效的大肠杆菌表达系统，用于生产重组MT-RNR2来源的Humanin，并验证其抗炎活性，为规模化生产奠定基础。
3. **"Humanin ameliorates insulin resistance via AMPK pathway activation"**
- 作者：Li, X. et al.
- 摘要：通过动物模型证明重组Humanin可通过激活AMPK信号通路改善胰岛素敏感性，提示其在糖尿病治疗中的潜在应用价值。
**实际研究建议**：
- MT-RNR2基因本身编码线粒体rRNA，但其反向转录产生的微肽Humanin是研究热点，建议以“Humanin”或“MT-RNR2 peptide”为关键词检索。
- 可查阅Nature Metabolism、Cell Death & Disease等期刊近年文献获取真实数据。
The mitochondrial MT-RNR2 gene, primarily known for encoding the 16S ribosomal RNA component of mitochondrial ribosomes, also harbors an open reading frame that produces a small bioactive peptide called Humanin (HN). Discovered serendipitously in 2001 during Alzheimer’s disease research, Humanin is a 24-amino-acid polypeptide with cytoprotective properties. It has garnered attention for its role in mitigating cellular stress, particularly in age-related pathologies. Humanin interacts with cell surface receptors, including the CNTFR/WSX-1/gp130 complex, activating downstream pathways like AKT and STAT3 to inhibit apoptosis, reduce oxidative stress, and enhance mitochondrial function.
Recombinant MT-RNR2-derived Humanin (rHN) is produced via genetic engineering in bacterial (e.g., E. coli) or mammalian expression systems, enabling scalable study of its therapeutic potential. Research highlights its neuroprotective effects in models of Alzheimer’s and Huntington’s diseases, as well as cardioprotective and metabolic benefits in diabetes and ischemia-reperfusion injury. Its small size and stability make it a promising candidate for drug development.
Interest also extends to its role in aging and longevity. Circulating Humanin levels decline with age and correlate with age-related disorders, suggesting diagnostic or prognostic utility. Variants like HNGF6A exhibit enhanced bioactivity, spurring structure-activity studies. Challenges remain in optimizing production yield, post-translational modifications, and targeted delivery in vivo. Current studies focus on elucidating its molecular mechanisms and evaluating preclinical efficacy, aiming to translate its cytoprotective properties into therapies for neurodegenerative diseases, metabolic syndromes, and inflammatory conditions.
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