| WB | 1/500 - 1/2000 | Human,Mouse,Monkey |
| IF | 咨询技术 | Human,Mouse,Monkey |
| IHC | 1/200 - 1/1000 | Human,Mouse,Monkey |
| ICC | 技术咨询 | Human,Mouse,Monkey |
| FCM | 咨询技术 | Human,Mouse,Monkey |
| Elisa | 1/10000 | Human,Mouse,Monkey |
| Aliases | OI1; OI2; OI3; OI4; EDSC; CAFYD; EDSARTH1; COL1A1; Collagen I |
| Entrez GeneID | 1277 |
| clone | 4C4 |
| WB Predicted band size | 139 kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Formulation | Purified antibody in PBS with 0.03% Proclin 300,0.5%BSA and 50% glycerol. |
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以下是3篇涉及LSD1/AOF2抗体的研究文献摘要信息，按发表时间排序：
1. **《LSD1 is a subunit of the NuRD complex and targets the metastasis programs in breast cancer》**
作者：Wang Y et al. (2009)
摘要：该研究开发了特异性识别LSD1/AOF2的单克隆抗体，验证其在染色质免疫共沉淀(ChIP)中的有效性，并发现LSD1通过NuRD复合体调控乳腺癌转移相关基因的表观遗传沉默。
2. **《A selective inhibitor of LSD1/KDM1A induces epigenetic reprogramming and differentiation in acute myeloid leukemia》**
作者：McGraw KL et al. (2013)
摘要：文献中采用商业化LSD1抗体(如Abcam ab17721)进行蛋白质印迹和免疫荧光实验，证明抑制LSD1活性可诱导白血病细胞分化，抗体特异性通过siRNA敲低实验验证。
3. **《LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells》**
作者：Adamo A et al. (2011)
摘要：研究使用兔源多克隆LSD1抗体(Sigma-Aldrich HPA027043)进行免疫沉淀和基因组定位分析，揭示LSD1通过调控多能性基因(如OCT4)的组蛋白去甲基化维持干细胞自我更新能力。
LSD1 (Lysine-Specific Demethylase 1), also known as AOF2 (Amine Oxidase Flavin-Containing Domain 2), is a histone-modifying enzyme that belongs to the flavin-dependent amine oxidase family. Discovered in 2004. it was the first histone demethylase identified, catalyzing the removal of methyl groups from mono- or dimethylated lysine residues, particularly H3K4me1/me2 and H3K9me1/me2. through an oxidation reaction. This activity enables LSD1/AOF2 to regulate gene expression by modulating chromatin structure, playing critical roles in embryonic development, cell differentiation, and cancer progression.
Antibodies targeting LSD1/AOF2 are widely used in epigenetics research to investigate its function in chromatin remodeling, transcriptional repression/activation, and interactions with protein complexes like CoREST or nuclear receptors. These antibodies are essential tools for techniques such as Western blotting, immunoprecipitation, ChIP-seq, and immunofluorescence. Specific LSD1/AOF2 antibodies may distinguish between post-translational modifications, isoforms, or binding states, aiding in studies of its dual role in oncogenesis (e.g., promoting leukemia or breast cancer) and tumor suppression. Researchers also utilize these antibodies to explore LSD1 inhibitors as potential therapeutics and to map its tissue-specific expression patterns in developmental or disease contexts.
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