| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FCRL5 |
| Uniprot No | Q96RD9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-844aa |
| 氨基酸序列 | QFARTPRPIIFLQPPWTTVFQGERVTLTCKGFRFYSPQKTKWYHRYLGKEILRETPDNILEVQESGEYRCQAQGSPLSSPVHLDFSSASLILQAPLSVFEGDSVVLRCRAKAEVTLNNTIYKNDNVLAFLNKRTDFHIPHACLKDNGAYRCTGYKESCCPVSSNTVKIQVQEPFTRPVLRASSFQPISGNPVTLTCETQLSLERSDVPLRFRFFRDDQTLGLGWSLSPNFQITAMWSKDSGFYWCKAATMPYSVISDSPRSWIQVQIPASHPVLTLSPEKALNFEGTKVTLHCETQEDSLRTLYRFYHEGVPLRHKSVRCERGASISFSLTTENSGNYYCTADNGLGAKPSKAVSLSVTVPVSHPVLNLSSPEDLIFEGAKVTLHCEAQRGSLPILYQFHHEGAALERRSANSAGGVAISFSLTAEHSGNYYCTADNGFGPQRSKAVSLSVTVPVSHPVLTLSSAEALTFEGATVTLHCEVQRGSPQILYQFYHEDMPLWSSSTPSVGRVSFSFSLTEGHSGNYYCTADNGFGPQRSEVVSLFVTVPVSRPILTLRVPRAQAVVGDLLELHCEAPRGSPPILYWFYHEDVTLGSSSAPSGGEASFNLSLTAEHSGNYSCEANNGLVAQHSDTISLSVIVPVSRPILTFRAPRAQAVVGDLLELHCEALRGSSPILYWFYHEDVTLGKISAPSGGGASFNLSLTTEHSGIYSCEADNGLEAQRSEMVTLKVAVPVSRPVLTLRAPGTHAAVGDLLELHCEALRGSPLILYRFFHEDVTLGNRSSPSGGASLNLSLTAEHSGNYSCEADNGLGAQRSETVTLYITGLTANR |
| 预测分子量 | 92.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FCRL5重组蛋白的3篇参考文献的简要总结：
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human FCRL5 Protein in B Cell Signaling*
**作者**：Wilson TJ, et al.
**摘要**：该研究通过哺乳动物表达系统成功制备了重组人源FCRL5蛋白，并验证其与B细胞受体(BCR)的协同作用。实验表明，FCRL5重组蛋白可增强BCR信号通路活性，提示其在调节B细胞免疫应答中的潜在功能。
2. **文献名称**：*Structural Insights into FCRL5 Interaction with IgA via Crystallographic Analysis*
**作者**：Hatzivassiliou G, et al.
**摘要**：通过X射线晶体学解析了重组FCRL5胞外域与IgA结合的复合物结构，揭示了其独特的结合界面和亲和力。该研究为FCRL5在黏膜免疫中的作用提供了分子机制依据。
3. **文献名称**：*FCRL5 Recombinant Protein as a Therapeutic Target in Autoantibody-Driven Diseases*
**作者**：Suzuki Y, et al.
**摘要**：研究利用重组FCRL5蛋白阻断小鼠模型中自身抗体的产生，发现其可显著减轻类风湿性关节炎症状，表明靶向FCRL5可能成为治疗自身免疫疾病的新策略。
(注：以上文献信息为示例性概括，实际引用需根据具体研究补充准确信息。)
**Background of FCRL5 Recombinant Protein**
FCRL5 (Fc receptor-like 5), a member of the Fc receptor-like (FCRL) family, is a transmembrane glycoprotein predominantly expressed on B lymphocytes. It shares structural homology with classical Fc receptors but lacks direct Fc-binding capacity. FCRL5 contains extracellular immunoglobulin (Ig) domains, a transmembrane region, and cytoplasmic tyrosine-based signaling motifs, enabling its role in modulating B-cell activation and immune regulation.
Functionally, FCRL5 interacts with undefined ligands and co-receptors to fine-tune B-cell receptor (BCR) signaling, influencing cell proliferation, differentiation, and antibody production. Its expression is dysregulated in autoimmune disorders (e.g., lupus, rheumatoid arthritis) and B-cell malignancies, highlighting its therapeutic and diagnostic potential.
Recombinant FCRL5 protein is engineered using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation, critical for structural and functional integrity. The protein is purified via affinity chromatography, yielding high-purity products for research applications.
In research, recombinant FCRL5 is used to study B-cell biology, immune tolerance, and disease mechanisms. It aids in ligand identification, antibody development, and screening for therapeutic agents targeting FCRL5-associated pathways. Additionally, it serves as an antigen in immunoassays to detect autoantibodies in autoimmune diseases.
As a therapeutic target, FCRL5 is explored for antibody-based therapies (e.g., monoclonal antibodies or CAR-T cells) to treat B-cell cancers or autoimmune conditions. Its restricted expression on B cells minimizes off-target effects, enhancing its appeal in precision medicine.
Overall, FCRL5 recombinant protein is a vital tool for unraveling immune regulation mechanisms and advancing targeted immunotherapies.
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