| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BRF1 |
| Uniprot No | Q92994 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-677aa |
| 氨基酸序列 | MTGRVCRGCGGTDIELDAARGDAVCTACGSVLEDNIIVSEVQFVESSGGGSSAVGQFVSLDGAGKTPTLGGGFHVNLGKESRAQTLQNGRRHIHHLGNQLQLNQHCLDTAFNFFKMAVSRHLTRGRKMAHVIAACLYLVCRTEGTPHMLLDLSDLLQVNVYVLGKTFLLLARELCINAPAIDPCLYIPRFAHLLEFGEKNHEVSMTALRLLQRMKRDWMHTGRRPSGLCGAALLVAARMHDFRRTVKEVISVVKVCESTLRKRLTEFEDTPTSQLTIDEFMKIDLEEECDPPSYTAGQRKLRMKQLEQVLSKKLEEVEGEISSYQDAIEIELENSRPKAKGGLASLAKDGSTEDTASSLCGEEDTEDEELEAAASHLNKDLYRELLGGAPGSSEAAGSPEWGGRPPALGSLLDPLPTAASLGISDSIRECISSQSSDPKDASGDGELDLSGIDDLEIDRYILNESEARVKAELWMRENAEYLREQREKEARIAKEKELGIYKEHKPKKSCKRREPIQASTAREAIEKMLEQKKISSKINYSVLRGLSSAGGGSPHREDAQPEHSASARKLSRRRTPASRSGADPVTSVGKRLRPLVSTQPAKKVATGEALLPSSPTLGAEPARPQAVLVESGPVSYHADEEADEEEPDEEDGEPCVSALQMMGSNDYGCDGDEDDGY |
| 预测分子量 | 75.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BRF1重组蛋白的3篇参考文献示例(文献信息为模拟虚构，仅供格式参考)：
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1. **文献名称**: *Recombinant Human BRF1 Expression and Purification for Structural Studies*
**作者**: Smith J, et al.
**摘要**: 本研究报道了人源BRF1重组蛋白在大肠杆菌中的高效表达与纯化方法，并通过X射线晶体学解析了其C端结构域的三维结构，揭示了其与RNA聚合酶III相互作用的分子机制。
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2. **文献名称**: *Functional Analysis of BRF1 in tRNA Transcription Using Reconstituted In Vitro Systems*
**作者**: Lee S, White R.
**摘要**: 利用重组BRF1蛋白与TFIIIB复合体进行体外重建实验，证明BRF1在RNA聚合酶III介导的tRNA基因转录起始中具有关键作用，并发现其磷酸化修饰可调控转录活性。
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3. **文献名称**: *BRF1 Overexpression in Cancer: Recombinant Protein-Based Screening for Inhibitors*
**作者**: Chen L, et al.
**摘要**: 通过构建BRF1重组蛋白的高通量筛选平台，鉴定了小分子化合物对BRF1异常活性的抑制作用，为靶向BRF1过表达的癌症治疗提供了潜在策略。
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(注：上述文献为示例，实际文献需通过PubMed/Google Scholar等平台检索关键词“BRF1 recombinant protein”或“BRF1 expression”获取。)
BRF1 (B-related factor 1), also known as TFIIB-related factor 1. is a critical component of the RNA polymerase III (Pol III) transcription machinery. It serves as a subunit of the TFIIIB complex, a conserved initiation factor required for the transcription of small non-coding RNAs, including tRNA, 5S rRNA, and other essential regulatory RNAs. BRF1 plays a pivotal role in recruiting RNA Pol III to gene promoters by bridging interactions between the TATA-binding protein (TBP) and promoter-bound transcription factors, such as TFIIIC. Its structural domains, including N-terminal and C-terminal extensions, enable precise promoter recognition and assembly of the transcription preinitiation complex.
Recombinant BRF1 protein is engineered through molecular cloning techniques, often expressed in bacterial or eukaryotic systems (e.g., E. coli, insect cells) for functional studies. Purified recombinant BRF1 retains its ability to bind DNA and interact with other transcription factors, making it a valuable tool for in vitro transcription assays, structural analyses (e.g., X-ray crystallography, cryo-EM), and investigations into Pol III regulation. Dysregulation of BRF1 is linked to diseases, including cancers and neurological disorders, due to altered RNA synthesis impacting cell proliferation and homeostasis. Research on recombinant BRF1 aids in elucidating its mechanistic roles in gene expression and exploring therapeutic strategies targeting Pol III activity. Its application extends to studying viral infections, as some pathogens hijack Pol III machinery for replication. Overall, BRF1 recombinant protein serves as a cornerstone for dissecting transcriptional regulation and its implications in health and disease.
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