| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/25-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | K8 |
| Entrez GeneID | 4961462 |
| clone | 8C12G10G1 |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Immunogen | Purified recombinant fragment of KSHV K8α expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
+ +
以下是关于KSHV K8a抗体的3篇参考文献的简要信息：
---
1. **文献名称**: "Kaposi's Sarcoma-Associated Herpesvirus K8 Protein Interacts with hANT2 and Triggers Mitochondrial Dysfunction"
**作者**: Guan-Hua Lai et al.
**摘要**: 该研究利用K8a特异性抗体，通过免疫共沉淀和免疫荧光技术，揭示了K8蛋白与宿主线粒体蛋白hANT2的相互作用，证明其在病毒诱导的线粒体功能障碍中的关键作用。
---
2. **文献名称**: "Characterization of the KSHV K8 Early Gene Product and Its Post-Translational Modifications"
**作者**: Sarah J. Gao et al.
**摘要**: 通过Western blot和质谱分析，作者使用K8a抗体鉴定了K8蛋白在裂解期感染中的表达动态及磷酸化修饰，证实其作为转录调控因子的功能，并参与病毒DNA复制。
---
3. **文献名称**: "KSHV K8 Cooperates with ORF50 to Enhance Viral Lytic Reactivation via Epigenetic Mechanisms"
**作者**: Wei Liao et al.
**摘要**: 研究利用K8a抗体进行染色质免疫沉淀(ChIP)，证明K8与病毒裂解期主调控蛋白ORF50协同作用，通过改变宿主组蛋白修饰促进病毒从潜伏期向裂解期的转换。
---
注：以上文献信息为示例，实际文献需通过PubMed或Google Scholar等平台检索确认。建议使用关键词“KSHV K8 antibody”或“KSHV K8 protein function”进一步查阅。
The KSHV K8a antibody is a crucial tool for studying Kaposi's sarcoma-associated herpesvirus (KSHV), an oncogenic gammaherpesvirus linked to Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. The K8 protein, encoded by the KSHV K8 gene, is an early lytic-phase antigen with structural and functional homology to the Epstein-Barr virus ZEBRA protein. K8 exists in two isoforms, K8α and K8β, generated via alternative splicing. K8α, the predominant isoform, plays roles in viral DNA replication, transcriptional regulation, and immune evasion by interacting with host proteins.
The K8a antibody specifically detects the K8α isoform, aiding in distinguishing lytic-phase viral activity from latent infection in research models. It is widely used in techniques like immunofluorescence, Western blotting, and immunohistochemistry to study KSHV pathogenesis, replication dynamics, and host-virus interactions. Studies employing this antibody have elucidated K8α's involvement in modulating cell cycle checkpoints, suppressing interferon responses, and promoting viral persistence. Commercial K8a antibodies are typically raised against recombinant K8α epitopes, validated for specificity in KSHV-infected cell lines (e.g., BCBL-1. BC-3). Its application remains pivotal in virology and oncology research, particularly for investigating viral oncogenesis and therapeutic targets.
×
