| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MRPL11 |
| Uniprot No | Q9Y3B7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-191aa |
| 氨基酸序列 | MSKLGRAARGLRKPEVGGVIRAIVRAGLAMPGPPLGPVLGQRGVSINQFCKEFNERTKDIKEGIPLPTKILVKPDRTFEIKIGQPTVSYFLKAAAGIEKGARQTGKEVAGLVTLKHVYEIARIKAQDEAFALQDVPLSSVVRSIIGSARSLGIRVVKDLSSEELAAFQKERAIFLAAQKEADLAAQEEAAK |
| 预测分子量 | 36.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MRPL11重组蛋白的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**: *"Recombinant expression and functional characterization of mitochondrial ribosomal protein L11 (MRPL11) in mammalian cells"*
**作者**: Zhang Y, et al.
**摘要**: 该研究成功构建了人源MRPL11的重组表达载体，并在HEK293细胞中实现高效表达。通过免疫共沉淀和蛋白质相互作用分析，发现MRPL11与线粒体核糖体大亚基的其他组分(如MRPL12和MRPL39)存在直接结合，提示其在核糖体组装中的关键作用。
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2. **文献名称**: *"Structural insights into MRPL11's role in mitochondrial translation fidelity"*
**作者**: Lee S, et al.
**摘要**: 本研究利用大肠杆菌表达系统纯化重组MRPL11蛋白，并通过X射线晶体学解析其三维结构。结构分析显示，MRPL11的C端结构域参与结合线粒体rRNA，并通过氢键网络维持翻译过程的准确性，为线粒体疾病相关突变位点(如Glu56Lys)的功能研究提供依据。
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3. **文献名称**: *"MRPL11 deficiency disrupts oxidative phosphorylation and induces apoptosis via impaired mitochondrial ribosome assembly"*
**作者**: Wang H, et al.
**摘要**: 通过构建MRPL11敲低细胞模型，并结合重组蛋白回补实验，证实MRPL11缺失导致线粒体核糖体组装异常、氧化磷酸化复合物减少及细胞凋亡。重组MRPL11的表达可部分恢复线粒体翻译功能，突显其作为潜在治疗靶点的价值。
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**备注**：上述文献为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议使用关键词“MRPL11 recombinant”、“mitochondrial ribosome assembly”或“MRPL11 structure”进行精准查询。
**Background of MRPL11 Recombinant Protein**
MRPL11 (Mitochondrial Ribosomal Protein L11) is a key component of the large subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondria, the energy-producing organelles, rely on their own translation machinery to synthesize subunits of the oxidative phosphorylation (OXPHOS) complexes, which are critical for ATP production. MRPL11. encoded by the nuclear *MRPL11* gene, is imported into mitochondria and assembled into the 39S ribosomal subunit. It plays a structural role in ribosome stability and facilitates the proper assembly of mitochondrial ribosomal complexes.
Dysregulation of MRPL11 is linked to mitochondrial dysfunction, contributing to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes. For instance, reduced MRPL11 expression impairs mitochondrial translation, leading to OXPHOS defects and increased reactive oxygen species (ROS), which may promote tumorigenesis or neuronal cell death.
Recombinant MRPL11 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its molecular interactions, structural features, and functional roles. This engineered protein retains post-translational modifications when expressed in eukaryotic systems, enabling accurate in vitro studies. Researchers utilize MRPL11 recombinant protein to investigate mitochondrial ribosome assembly mechanisms, screen for potential therapeutic compounds targeting mitochondrial disorders, or explore its role in cellular stress responses.
Recent studies also highlight MRPL11's potential as a biomarker for certain cancers, as its expression levels correlate with disease progression. Overall, MRPL11 recombinant protein serves as a vital tool for advancing our understanding of mitochondrial biology and its implications in human health and disease.
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