| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOP58 |
| Uniprot No | Q9Y2X3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-529aa |
| 氨基酸序列 | MLVLFETSVGYAIFKVLNEKKLQEVDSLWKEFETPEKANKIVKLKHFEKFQDTAEALAAFTALMEGKINKQLKKVLKKIVKEAHEPLAVADAKLGGVIKEKLNLSCIHSPVVNELMRGIRSQMDGLIPGVEPREMAAMCLGLAHSLSRYRLKFSADKVDTMIVQAISLLDDLDKELNNYIMRCREWYGWHFPELGKIISDNLTYCKCLQKVGDRKNYASAKLSELLPEEVEAEVKAAAEISMGTEVSEEDICNILHLCTQVIEISEYRTQLYEYLQNRMMAIAPNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIYHASLVGQTSPKHKGKISRMLAAKTVLAIRYDAFGEDSSSAMGVENRAKLEARLRTLEDRGIRKISGTGKALAKTEKYEHKSEVKTYDPSGDSTLPTCSKKRKIEQVDKEDEITEKKAKKAKIKVKVEEEEEEKVAEEEETSVKKKKKRGKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED |
| 预测分子量 | 75.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOP58重组蛋白的3篇参考文献示例(注：以下内容为模拟示例，实际文献需根据具体研究查询)：
1. **文献名称**: "Structural insights into NOP58 interactions within the box C/D snoRNP complex"
**作者**: Smith J, et al.
**摘要**: 本研究解析了重组NOP58蛋白与snoRNA核心结构域的晶体结构，揭示了其通过α-螺旋结构域与fibrillarin及NOP56的结合机制，为snoRNP复合体的组装提供了分子基础。
2. **文献名称**: "NOP58 mutations impair ribosome biogenesis and cause Diamond-Blackfan anemia"
**作者**: Chen L, et al.
**摘要**: 通过重组NOP58蛋白功能实验，发现其特定突变会破坏rRNA前体的甲基化修饰，导致核糖体合成缺陷，阐明了该基因突变与先天性骨髓衰竭疾病的关联机制。
3. **文献名称**: "Recombinant NOP58 expression in E. coli and its role in telomerase regulation"
**作者**: Wang Y, et al.
**摘要**: 报道了利用大肠杆菌系统高效表达可溶性NOP58重组蛋白的方法，并证明其通过与端粒酶RNA组分互作调控端粒延长，为癌症靶向研究提供了工具。
4. **文献名称**: "Proteomic analysis of NOP58-containing complexes in human cells"
**作者**: Gonzalez A, et al.
**摘要**: 使用重组NOP58抗体进行免疫沉淀-MS分析，鉴定出多个新型互作蛋白(如NHP2、TCAB1)，揭示了其在细胞内转运及RNA加工中的扩展功能网络。
**Background of NOP58 Recombinant Protein**
NOP58. a core component of the box C/D small nucleolar ribonucleoprotein (snoRNP) complex, plays a critical role in ribosomal RNA (rRNA) biogenesis. It facilitates the site-specific 2′-O-methylation of precursor rRNA (pre-rRNA), a post-transcriptional modification essential for ribosome assembly, stability, and function. Structurally, NOP58 contains conserved N-terminal and C-terminal domains that mediate interactions with other snoRNP proteins, including NOP56. fibrillarin (the methyltransferase), and the noncoding RNA component.
Recombinant NOP58 protein is engineered for in vitro studies to dissect its molecular interactions, structural dynamics, and regulatory mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains required for snoRNP assembly and enzymatic activity. Researchers utilize this tool to investigate diseases linked to rRNA processing defects, such as certain cancers, neurodegenerative disorders, and ribosomopathies. For example, mutations in NOP58-associated genes are implicated in dyskeratosis congenita and Prader-Willi syndrome, highlighting its clinical relevance.
Studies employing recombinant NOP58 also explore its role in cell cycle regulation and stress responses, as snoRNP dysfunction can trigger p53-dependent pathways. Additionally, it serves as a target for drug discovery aimed at modulating ribosome biogenesis in hyperproliferative diseases. Technical applications include pull-down assays, structural analysis (e.g., crystallography or cryo-EM), and functional reconstitution of snoRNP complexes.
Overall, NOP58 recombinant protein is a vital resource for advancing our understanding of ribosome biology and its pathological disruptions.
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