| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPIAL4A |
| Uniprot No | Q9Y536 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-164aa |
| 氨基酸序列 | MVNSVVFFDITVDGKPLGRISIKLFADKILKTAENFRALSTGEKGFRYKGSCFHRIIPGFMCQGGDFTRHNGTGDKSIYGEKFDDENLIRKHTGSGILSMANAGPNTNGSQFFICAAKTEWLDGKHVAFGKVKERVNIVEAMEHFGYRNSKTSKKITIADCGQF |
| 预测分子量 | 34.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPIAL4A重组蛋白的3篇参考文献的示例(注：部分内容为假设性概括，实际文献需根据具体数据库检索)：
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1. **文献名称**: *"Recombinant PPIAL4A Expression and Its Role in Cellular Proliferation"*
**作者**: Zhang Y, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组PPIAL4A蛋白，验证了其作为肽酰脯氨酰异构酶的功能。实验表明，PPIAL4A通过调控细胞周期相关蛋白的折叠，促进肿瘤细胞的增殖，提示其可能成为癌症治疗的潜在靶点。
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2. **文献名称**: *"Structural Characterization of PPIAL4A and Interaction with HIV-1 Capsid Protein"*
**作者**: Lee S, Kim JH.
**摘要**: 通过X射线晶体学解析了PPIAL4A重组蛋白的三维结构，发现其与HIV-1衣壳蛋白存在特异性结合。该相互作用可能影响病毒复制，为抗病毒药物设计提供了新思路。
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3. **文献名称**: *"PPIAL4A Recombinant Protein as a Biomarker in Autoimmune Diseases"*
**作者**: Müller R, et al.
**摘要**: 研究检测了类风湿性关节炎患者血清中PPIAL4A的水平，发现其表达显著升高。通过重组蛋白的体外实验证实，PPIAL4A可能通过激活NF-κB通路加剧炎症反应，提示其作为疾病标志物的潜力。
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**提示**：实际研究中，PPIAL4A的直接文献可能较少，建议拓展检索其同源蛋白(如PPIA)或结合“重组蛋白表达技术”等关键词。建议使用PubMed或Web of Science获取最新数据。
**Background on PPIAL4A Recombinant Protein**
PPIAL4A (Peptidylprolyl Isomerase A Like 4A) is a member of the cyclophilin protein family, characterized by its peptidylprolyl isomerase (PPIase) activity, which facilitates protein folding by catalyzing the cis-trans isomerization of proline residues. While its exact biological role remains under investigation, PPIAL4A shares structural and functional homology with cyclophilin A (PPIA), a well-studied protein involved in immune regulation, apoptosis, and cellular stress responses. PPIAL4A is encoded by the *PPIAL4A* gene, located on human chromosome 1. and is expressed in various tissues, though its expression patterns and regulation are less characterized compared to other cyclophilin family members.
Recombinant PPIAL4A protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research applications. Its recombinant production enables studies on its enzymatic activity, interaction partners, and potential roles in cellular processes such as protein trafficking, signal transduction, or viral replication, where cyclophilins are known to participate. Notably, cyclophilin inhibitors (e.g., cyclosporine A) are used clinically, highlighting the therapeutic relevance of understanding PPIAL4A’s functions.
Research on PPIAL4A may provide insights into its unique contributions to cyclophilin-mediated pathways, particularly in contexts like cancer, neurodegeneration, or infectious diseases. However, limited literature specifically addressing PPIAL4A underscores the need for further exploration to clarify its physiological and pathological significance. Current studies focus on characterizing its enzymatic kinetics, subcellular localization, and tissue-specific expression to differentiate it from other cyclophilin isoforms. Recombinant PPIAL4A thus serves as a critical tool for unraveling its distinct biological roles and potential as a therapeutic target.
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